Characterization of the Grp94/OS-9 chaperone-lectin complex.
Characterization of the Grp94/OS-9 chaperone-lectin complex.
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Grp94/OS-9 伴侣-凝集素复合物的表征。
DOI：
10.1016/j.jmb.2014.08.024
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发表时间：
2014
影响因子：
5.6
通讯作者：
Gewirth,DanielT
中科院分区：
文献类型：
--
作者：
Seidler,PaulM;Shinsky,StephenA;Hong,Feng;Li,Zihai;Cosgrove,MichaelS;Gewirth,DanielT
Grp94 is a macromolecular chaperone belonging to the hsp90 family and is the most abundant glycoprotein in the endoplasmic reticulum (ER) of mammals. In addition to its essential role in protein folding, Grp94 was proposed to participate in the ER-associated degradation quality control pathway by interacting with the lectin OS-9, a sensor for terminally misfolded proteins. To understand how OS-9 interacts with ER chaperone proteins, we mapped its interaction with Grp94. Glycosylation of the full-length Grp94 protein was essential for OS-9 binding, although deletion of the Grp94 N-terminal domain relieved this requirement suggesting that the effect was allosteric rather than direct. Although yeast OS-9 is composed of a well-established N-terminal mannose recognition homology lectin domain and a C-terminal dimerization domain, we find that the C-terminal domain of OS-9 in higher eukaryotes contains “mammalian-specific insets” that are specifically recognized by the middle and C-terminal domains of Grp94. Additionally, the Grp94 binding domain in OS-9 was found to be intrinsically disordered. The biochemical analysis of the interacting regions provides insight into the manner by which the two associate and it additionally hints at a plausible biological role for the Grp94/OS-9 complex.
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DOI：
10.1016/j.bbamcr.2011.10.013
发表时间：
2012-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Marzec M;Eletto D;Argon Y
通讯作者：
Argon Y
HSP90-TAU复合物揭示了伴侣作用特异性的分子基础。
影响因子：
64.5
作者：
Karagöz GE;Duarte AM;Akoury E;Ippel H;Biernat J;Morán Luengo T;Radli M;Didenko T;Nordhues BA;Veprintsev DB;Dickey CA;Mandelkow E;Zweckstetter M;Boelens R;Madl T;Rüdiger SG
通讯作者：
Rüdiger SG
人 0S-9 是糖蛋白内质网相关降解所需的一种凝集素，可识别甘露糖修饰的 N-聚糖
DOI：
--
发表时间：
2009
期刊：
J. Biol. Chem. 284(25)
影响因子：
--
作者：
N. Hosokawa;Y. Kamiya;D. Kamiya;K. Kato;K. Nagata
通讯作者：
K. Nagata
影响因子：
16
作者：
Street TO;Lavery LA;Agard DA
通讯作者：
Agard DA
影响因子：
16.6
作者：
Liu, Bei;Yang, Yi;Qiu, Zhijuan;Staron, Matthew;Hong, Feng;Li, Yi;Wu, Shuang;Li, Yunfeng;Hao, Bing;Bona, Robert;Han, David;Li, Zihai
通讯作者：
Li, Zihai