Regulation of Macrophage Polarization by miR-449a/Cripto-1-PI3K/AKT/NF-κB Signaling Pathway in Allogeneic Transfusion Mice.
Regulation of Macrophage Polarization by miR-449a/Cripto-1-PI3K/AKT/NF-κB Signaling Pathway in Allogeneic Transfusion Mice.
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DOI：
10.1155/2023/1277258
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发表时间：
2023
影响因子：
--
通讯作者：
Guo, Jian-Rong
中科院分区：
文献类型：
--
作者：
Wang, Huan;Yao, Na;Wu, Man-di;Yue, Ke;Bai, Yu;You, Lai-Wei;Liu, Tong;Xu, Fang;Guo, Jian-Rong
本研究通过构建小鼠异体输血模型，分析同种异体输血后巨噬细胞极化在免疫应答中的作用及Cripto-1的表达。为了分析miR-449a在正常和异常情况下对PI3K/AKT/NF-κB信号通路及下游相关调控因子表达的影响，我们分别采用了体外和体内实验。通过阻断或激活基因表达和western blotting分析PI3K/AKT/NF-κB信号通路的分子机制。体外实验证实，抑制miR-449a可增加Cripto-1蛋白的表达。体内实验证实，异体输血降低了Cripto-1的表达，通过AKT/PI3K磷酸化进一步抑制NF-κB信号通路，调节巨噬细胞极化，抑制巨噬细胞M1极化，促进M2极化，从而影响机体的免疫应答。
In this study, the expression of Cripto-1 and the role of macrophage polarization in immune response after allogeneic transfusion were analyzed by constructing a mouse model of allogeneic transfusion. In order to analyze the effects of miR-449a on the PI3K/AKT/NF-κB signaling pathway and the expression of downstream related regulatory factors under normal and abnormal conditions, we adopt in vitro and in vivo experiments separately. The molecular mechanism of PI3K/AKT/NF-κB signaling pathway was analyzed by blocking or activating gene expression and western blotting. Experiment in vitro has confirmed that inhibition of miR-449a increased the protein expression of Cripto-1. In vivo experiments confirmed that allogeneic transfusion reduced the expression of Cripto-1, which further inhibited NF-κB signaling pathway through AKT/PI3K phosphorylation, regulated macrophage polarization, inhibited M1 polarization of macrophages, promoted M2 polarization, and thus affected immune response of the body.
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影响因子：
5.7
作者：
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通讯作者：
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影响因子：
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