A barrier to homologous recombination between sympatric strains of the cooperative soil bacterium Myxococcus xanthus.
A barrier to homologous recombination between sympatric strains of the cooperative soil bacterium Myxococcus xanthus.
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DOI：
10.1038/ismej.2016.34
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发表时间：
2016-10
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影响因子：
--
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中科院分区：
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--
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黄色粘球菌在土壤中滑动寻找猎物微生物，但当食物来源耗尽时，细胞会在多细胞子实体内合作构建并形成孢子。之前从一块 16 × 16 厘米大小的土壤中分离出的 M. xanthus 菌株已被证明已多样化为许多不同的相容性类型，其特征是蜂群菌落在相遇时无法合并。我们对来自该群体的 22 个分离株的基因组进行了测序，这些分离株属于两个最常出现的多位点序列类型 (MLST) 分支，以追踪早期基因组分歧的模式，特别是与社会分歧相关的模式。尽管同源重组在两个 MLST 进化枝内频繁发生，但我们发现它们之间几乎完全不存在重组事件。由于这两个分支关系非常密切并且共生，生态或遗传障碍必然会减少它们之间的遗传交换。我们发现辅助基因组的变化率大于核心基因组的氨基酸取代率。我们确定了一个大的基因组区域，该基因组区域在不能自由合并的分离株之间始终存在差异，因此是隐藏负责自我/非自我歧视的基因的候选区域。
The bacterium Myxococcus xanthus glides through soil in search of prey microbes, but when food sources run out, cells cooperatively construct and sporulate within multicellular fruiting bodies. M. xanthus strains isolated from a 16 × 16-cm-scale patch of soil were previously shown to have diversified into many distinct compatibility types that are distinguished by the failure of swarming colonies to merge upon encounter. We sequenced the genomes of 22 isolates from this population belonging to the two most frequently occurring multilocus sequence type (MLST) clades to trace patterns of incipient genomic divergence, specifically related to social divergence. Although homologous recombination occurs frequently within the two MLST clades, we find an almost complete absence of recombination events between them. As the two clades are very closely related and live in sympatry, either ecological or genetic barriers must reduce genetic exchange between them. We find that the rate of change in the accessory genome is greater than the rate of amino-acid substitution in the core genome. We identify a large genomic tract that consistently differs between isolates that do not freely merge and therefore is a candidate region for harbouring gene(s) responsible for self/non-self discrimination.
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影响因子：
6.7
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Auer M