Identification of differentially expressed genes associated with differential body size in mandarin fish (Siniperca chuatsi)
Identification of differentially expressed genes associated with differential body size in mandarin fish (Siniperca chuatsi)
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鳜鱼体型差异相关差异表达基因的鉴定
DOI：
10.1007/s10709-016-9913-2
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发表时间：
2016-07
期刊：
影响因子：
1.5
通讯作者：
Song Yi
中科院分区：
文献类型：
--
作者：
Guo Wenjie;Lv Liyuan;Wang Qingchao;Song Yi
体型大小是鱼类的一个明显而重要的特征。鳜鱼Sinia chuatsi（Basilewsky）是我国广泛养殖的鲈形目鱼类之一。鳜鱼个体大小的差异是常见的，对养殖生产有重要影响。然而，目前对其遗传控制知之甚少。本研究以不同体型的鳜鱼为研究对象，分别在孵化后30天和180天进行数字化基因表达谱分析和转录组测序。在30和180 dph时，大规格鱼的体重、全长和体长均显著高于小规格鱼（P< 0.05）。在30和180 dph下，分别在小型鱼和大型鱼之间鉴定了2171和2014个差异表达基因。RT定量PCR（qPCR）分析表明，经过相同训练程序的鳜鱼中，10个筛选基因的表达差异较大。这些基因涉及生长激素-胰岛素样生长因子轴、细胞增殖和分化、食欲控制、葡萄糖代谢、生殖和性别大小二型途径。本研究将有助于全面了解鳜鱼个体大小调控的复杂性，并为今后的研究提供有价值的信息。
Body size is an obvious and important characteristic of fish. Mandarin fishSiniperca chuatsi(Basilewsky) is one of the most valuable perciform species widely cultured in China. Individual differences in body size are common in mandarin fish and significantly influence the aquaculture production. However, little is currently known about its genetic control. In this study, digital gene expression profiling and transcriptome sequencing were performed in mandarin fish with differential body size at 30 and 180 days post-hatch (dph), respectively. Body weight, total length and body length of fish with big-size were significantly higher than those with small-size at both 30 and 180 dph (P< 0.05). 2171 and 2014 differentially expressed genes were identified between small-size and big-size fish at 30 and 180 dph, respectively. RT quantitative PCR (qPCR) analysis showed that the differential expression of 10 selected genes in mandarin fish that went through the same training procedure. The genes were involved in the growth hormone–insulin-like growth factor axis, cell proliferation and differentiation, appetite control, glucose metabolism, reproduction and sexual size dimorphism pathways. This study will help toward a comprehensive understanding of the complexity of regulation of body size in mandarin fish individuals and provide valuable information for future research.
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影响因子：
1.5
作者：
Tan, Yinfei;Dourdin, Nathalie;Greer, Peter A.
通讯作者：
Greer, Peter A.
影响因子：
3.7
作者：
Salem M;Vallejo RL;Leeds TD;Palti Y;Liu S;Sabbagh A;Rexroad CE 3rd;Yao J
通讯作者：
Yao J
DOI：
--
发表时间：
2006
期刊：
Freshwater Fisheries
影响因子：
--
作者：
Wang Xiao-qing
通讯作者：
Wang Xiao-qing
DOI：
10.4238/2011.december.12.1
发表时间：
2011-12
期刊：
Genetics and molecular research : GMR
影响因子：
--
作者：
Qian Ma;Shufang Liu;Z.-M. Zhuang;Lin Lin-Lin;Z. Sun;C. Liu;Y. Su;Q. Tang
通讯作者：
Qian Ma;Shufang Liu;Z.-M. Zhuang;Lin Lin-Lin;Z. Sun;C. Liu;Y. Su;Q. Tang
影响因子：
4.4
作者：
Danzmann RG;Kocmarek AL;Norman JD;Rexroad CE 3rd;Palti Y
通讯作者：
Palti Y