Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.
Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.
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DOI：
10.1039/c6tb00331a
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发表时间：
2016-05-28
期刊：
影响因子：
--
通讯作者：
Hay DC
中科院分区：
文献类型：
--
作者：
Lucendo-Villarin B;Rashidi H;Cameron K;Hay DC
多能干细胞衍生的肝细胞（肝细胞）代表了用于生物学和临床应用的原代组织的有希望的替代物。多能干细胞衍生的肝细胞（肝细胞）代表了用于生物学和临床应用的原代组织的有希望的替代物。迄今为止，大多数肝细胞维持和分化系统依赖于使用动物源性组分。这是干细胞衍生肝细胞大规模生产和应用的重要障碍。最近，使用确定的生物制剂克服了二维单层培养的这些限制。为了进一步改善细胞表型，三维培养系统已被用来更好地模拟体内情况，借鉴材料化学，工程和生物学。在这篇综述中，我们讨论了在该领域的努力，在规定的条件下分化多能干细胞向肝细胞。
Pluripotent stem cell derived liver cells (hepatocytes) represent a promising alternative to primary tissue for biological and clinical applications. Pluripotent stem cell derived liver cells (hepatocytes) represent a promising alternative to primary tissue for biological and clinical applications. To date, most hepatocyte maintenance and differentiation systems have relied upon the use of animal derived components. This serves as a significant barrier to large scale production and application of stem cell derived hepatocytes. Recently, the use of defined biologics has overcome those limitations in two-dimensional monolayer culture. In order to improve the cell phenotype further, three-dimensional culture systems have been employed to better mimic the in vivo situation, drawing upon materials chemistry, engineering and biology. In this review we discuss efforts in the field, to differentiate pluripotent stem cells towards hepatocytes under defined conditions.
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DOI：
10.1002/adma.201501351
发表时间：
2015-07-15
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Celiz AD;Smith JG;Patel AK;Hook AL;Rajamohan D;George VT;Flatt L;Patel MJ;Epa VC;Singh T;Langer R;Anderson DG;Allen ND;Hay DC;Winkler DA;Barrett DA;Davies MC;Young LE;Denning C;Alexander MR
通讯作者：
Alexander MR
影响因子：
15.9
作者：
BISSELL, DM;ARENSON, DM;ROLL, FJ
通讯作者：
ROLL, FJ
影响因子：
4.1
作者：
Brolen, Gabriella;Sivertsson, Louise;Heins, Nico
通讯作者：
Heins, Nico
影响因子：
14
作者：
Brafman, David A.;Chang, Chien W.;Fernandez, Antonio;Willert, Karl;Varghese, Shyni;Chien, Shu
通讯作者：
Chien, Shu
影响因子：
5.2
作者：
Agarwal, Sadhana;Holton, Katherine L.;Lanza, Robert
通讯作者：
Lanza, Robert