Genomic and functional analyses of the gentisate and protocatechuate ring-cleavage pathways and related 3-hydroxybenzoate and 4-hydroxybenzoate peripheral pathways in Burkholderia xenovorans LB400.
Genomic and functional analyses of the gentisate and protocatechuate ring-cleavage pathways and related 3-hydroxybenzoate and 4-hydroxybenzoate peripheral pathways in Burkholderia xenovorans LB400.
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DOI：
10.1371/journal.pone.0056038
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Seeger M
中科院分区：
文献类型：
--
作者：
Romero-Silva MJ;Méndez V;Agulló L;Seeger M
本研究通过基因组学和功能学方法分析了异种伯克霍尔德菌LB400中龙胆酸和原儿茶酸途径，并提出了它们在3-羟基苯甲酸酯（3-HBA）和4-羟基苯甲酸酯（4-HBA）降解中的作用。LB400基因组具有两个相同的mhbRTDHI基因簇，编码龙胆酸途径，一个mhbM基因簇编码3-HBA 6-羟化酶，将3-HBA转化为龙胆酸。编码原儿茶酸途径的pca基因和编码将4-HBA氧化为原儿茶酸的4-HBA 3-单加氧酶的pobA基因以基因簇和单基因的形式排列在次要染色体上，但也存在于主染色体和巨质粒上。菌株LB400能在gentisate、protocatechaate、3-HBA和4-HBA上生长。转录分析表明，编码龙胆酸1,2-双加氧酶的mhbD基因在3- hba、4-HBA和龙胆酸的生长过程中表达，而编码原儿茶酸3,4-双加氧酶的pcaG基因仅在4-HBA和原儿茶酸的生长过程中表达。在菌株LB400中，编码3-HBA 6-羟化酶的mhbM基因在HBAs、龙胆酸盐、原儿茶酸盐和葡萄糖的生长过程中被转录。编码4-HBA 3-单加氧酶的pobA基因在hba和葡萄糖的生长过程中表达。3- hba和4- hba培养的LB400细胞显示龙胆酸1,2-双加氧酶活性，而原儿茶酸3,4-双加氧酶活性仅在4- hba培养的细胞中观察到。mhbR基因编码的marr型转录调控因子可能调控MhbT转运体的表达，而pcaQ和pcaR基因编码的lysr型转录调控因子分别调控pcaHG和pcaIJBDC基因，在HBAs、gentisate、原儿茶酸盐和葡萄糖的生长过程中都被转录，表明它们具有基础组成表达。结果表明，异种芽孢杆菌LB400中有活性的龙胆酸、原儿茶酸、3-HBA和4-HBA的分解代谢途径，表明3-HBA仅通过龙胆酸途径通道，而4-HBA则进入原儿茶酸中枢途径，并可能进入龙胆酸途径。
In this study, the gentisate and protocatechuate pathways in Burkholderia xenovorans LB400 were analyzed by genomic and functional approaches, and their role in 3-hydroxybenzoate (3-HBA) and 4-hydroxybenzoate (4-HBA) degradation was proposed. The LB400 genome possesses two identical mhbRTDHI gene clusters encoding the gentisate pathway and one mhbM gene encoding a 3-HBA 6-hydroxylase that converts 3-HBA into gentisate. The pca genes encoding the protocatechuate pathway and the pobA gene encoding the 4-HBA 3-monooxygenase that oxidizes 4-HBA into protocatechuate are arranged in gene clusters and single genes mainly at the minor chromosome, but also at the major chromosome and the megaplasmid. Strain LB400 was able to grow on gentisate, protocatechuate, 3-HBA and 4-HBA. Transcriptional analyses showed that the mhbD gene encoding the gentisate 1,2-dioxygenase was expressed during growth on 3-HBA, 4-HBA and gentisate, whereas the pcaG gene encoding the protocatechuate 3,4-dioxygenase was expressed only during growth on 4-HBA and protocatechuate. The mhbM gene encoding the 3-HBA 6-hydroxylase was transcribed in strain LB400 during growth on HBAs, gentisate, protocatechuate and glucose. The pobA gene encoding the 4-HBA 3-monooxygenase was expressed during growth on HBAs and glucose. 3-HBA- and 4-HBA-grown LB400 cells showed gentisate 1,2-dioxygenase activity, whereas protocatechuate 3,4-dioxygenase activity was observed only in 4-HBA-grown cells. The mhbR gene encoding a MarR-type transcriptional regulator that probably regulates the expression of the MhbT transporter, and the pcaQ and pcaR genes encoding LysR-type transcriptional regulators that regulate pcaHG and pcaIJBDC genes, respectively, were transcribed during growth on both HBAs, gentisate, protocatechuate and glucose, suggesting a basal constitutive expression. The results indicate active gentisate, protocatechuate, 3-HBA and 4-HBA catabolic pathways in B. xenovorans LB400 and suggest that 3-HBA is channeled exclusively through the gentisate route, whereas 4-HBA is funneled into the protocatechuate central pathway and potentially into the gentisate pathway.
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影响因子：
3.2
作者：
HARTNETT, C;NEIDLE, EL;ORNSTON, LN
通讯作者：
ORNSTON, LN
影响因子：
8
作者：
Perera, Inoka C.;Grove, Anne
通讯作者：
Grove, Anne
DOI：
10.1073/pnas.0606924103
发表时间：
2006-10-17
影响因子：
11.1
作者：
Chain, Patrick S. G.;Denef, Vincent J.;Tiedje, James M.
通讯作者：
Tiedje, James M.
影响因子：
3.2
作者：
CRAWFORD, RL
通讯作者：
CRAWFORD, RL
影响因子：
5.1
作者：
Jiménez, JI;Miñambres, B;Díaz, E
通讯作者：
Díaz, E