A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression, amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice.
A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression, amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice.
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DOI：
10.1016/j.jnutbio.2018.06.007
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Zempleni J
中科院分区：
文献类型：
--
作者：
Leiferman A;Shu J;Grove R;Cui J;Adamec J;Zempleni J
外泌体是将货物从供体细胞转移到受体细胞的纳米颗粒，在那里它们引起基因表达和代谢的变化。有证据表明，外泌体及其货物也从饮食来源如牛乳中吸收，并且牛外泌体促进鼠C2 C12肌管细胞培养物中肌纤维的生长。本研究的目的是确定牛乳外泌体的饮食摄入是否会改变小鼠骨骼肌的强度，基因表达和氨基酸谱。三周龄的雄性和雌性C57 BL/6小鼠喂食基于AIN 93 G的外泌体和RNA耗尽（ERD）饮食六周;对照喂食外泌体和RNA充足（ERS）饮食。通过代谢笼、握力分析仪、临床化学分析仪、靶向LC/MS-MS和RNA测序分析，分析了摄食行为、代谢、握力、肝肾功能、氨基酸谱和基因表达模式的变量。饮食对食物和水的摄入量，呼吸交换率，体力活动，握力，肝肾功能障碍的标志物和肌肉中的氨基酸谱没有影响。只有12个和9个mRNA差异表达的骨骼肌雌性和雄性小鼠，分别喂食ERD和ERS饮食。ERD饮食对骨骼肌中基因表达和游离氨基酸水平的适度影响与牛乳外泌体及其货物在骨骼肌以外的组织中积累的观察结果一致。
Exosomes are nanoparticles that transfer cargos from donor cells to recipient cells where they elicit changes in gene expression and metabolism. Evidence suggests that exosomes and their cargos are also absorbed from dietary sources such as bovine milk, and bovine exosomes promote the growth of myofibers in murine C2C12 myotube cell cultures. The aim of the current study was to determine whether the dietary intake of bovine milk exosomes alters strength, gene expression and amino acid profiles in murine skeletal muscles. Male and female C57BL/6 mice, age three weeks, were fed an AIN93G-based, exosome and RNA-depleted (ERD) diet for six weeks; controls were fed an exosome and RNA-sufficient (ERS) diet. Variables of feeding behavior, metabolism, grip strength, liver and kidney function, amino acid profiles, and gene expression patterns were analyzed by using metabolic cages, grip strength analyzers, clinical chemistry analyzers, targeted LC/MS-MS, and RNA sequencing analysis. The diets had no effect on food and water intake, respiratory exchange rate, physical activity, grip strength, markers of liver and kidney dysfunction, and amino acid profiles in muscle. Only twelve and nine mRNAs were differentially expressed in skeletal muscle from female and male mice, respectively, fed ERD and ERS diets. The modest effect of the ERD diet on gene expression and levels of free amino acids in skeletal muscle is consistent with observations that bovine milk exosomes and their cargos accumulate in tissues other than skeletal muscle.
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作者：
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DOI：
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影响因子：
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作者：
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DOI：
10.1126/science.1215691
发表时间：
2012-04-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
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