Protection against Borreliella burgdorferi infection mediated by a synthetically engineered DNA vaccine.
Protection against Borreliella burgdorferi infection mediated by a synthetically engineered DNA vaccine.
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DOI：
10.1080/21645515.2020.1789408
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发表时间：
2020-09-01
影响因子：
4.8
通讯作者：
Smith TRF
中科院分区：
文献类型：
--
作者：
Guibinga GH;Sahay B;Brown H;Cooch N;Chen J;Yan J;Reed C;Mishra M;Yung B;Pugh H;Schultheis K;Esquivel RN;Weiner DB;Humeau LH;Broderick KE;Smith TRF
莱姆病是北美最常见的病媒传播疾病。病原体是伯氏疏螺旋体，通过硬蜱传播给哺乳动物宿主。近年来，莱姆病的病例有所增加。目前，市场上还没有人用疫苗。我们描述了一种新的合成工程DNA疫苗，pLD 1靶向外表面蛋白A（OspA）的伯氏疏螺旋体的发展。用pLD 1免疫C3 H/HeN小鼠激发了强烈的体液和细胞免疫应答，其赋予针对活伯氏疏螺旋体细菌攻击的完全保护。我们还评估了Hartley豚鼠皮内（ID）递送pLD 1，证明诱导了持续至少1年的稳健和持久的体液免疫。我们通过显示靶向与保护相关的OspA表位的抗体在免疫豚鼠中显著升高来提供pLD 1效力的证据。所描述的研究为pDL 1作为莱姆病控制的潜在疫苗的进展提供了基础。
Lyme disease is the most common vector-borne disease in North America. The etiological agent is the spirochete Borreliella burgdorferi, transmitted to mammalian hosts by the Ixodes tick. In recent years there has been an increase in the number of cases of Lyme disease. Currently, there is no vaccine on the market for human use. We describe the development of a novel synthetically engineered DNA vaccine, pLD1 targeting the outer-surface protein A (OspA) of Borreliella burgdorferi. Immunization of C3 H/HeN mice with pLD1 elicits robust humoral and cellular immune responses that confer complete protection against a live Borreliella burgdorferi bacterial challenge. We also assessed intradermal (ID) delivery of pLD1 in Hartley guinea pigs, demonstrating the induction of robust and durable humoral immunity that lasts at least 1 year. We provide evidence of the potency of pLD1 by showing that antibodies targeting the OspA epitopes which have been associated with protection are prominently raised in the immunized guinea pigs. The described study provides the basis for the advancement of pDL1 as a potential vaccine for Lyme disease control.
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影响因子：
6.2
作者：
Bode C;Zhao G;Steinhagen F;Kinjo T;Klinman DM
通讯作者：
Klinman DM
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影响因子：
7
作者：
Sardesai NY;Weiner DB
通讯作者：
Weiner DB
影响因子：
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作者：
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影响因子：
3.1
作者：
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通讯作者：
Fikrig, E
影响因子：
3.1
作者：
Golde, WT;Piesman, J;Frantz, JC
通讯作者：
Frantz, JC