Analysis of telomerase activity based on a spired DNA tetrahedron TS primer.
Analysis of telomerase activity based on a spired DNA tetrahedron TS primer.
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基于螺旋 DNA 四面体 TS 引物的端粒酶活性分析。
DOI：
10.1016/j.bios.2014.08.056
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发表时间：
2015-05
影响因子：
12.6
通讯作者：
Jia, Nengqin
中科院分区：
文献类型：
--
作者：
Fan, Chunhai;Huang, Qing;Liu, Gang;Jia, Nengqin
端粒引物的可及性和酶反应空间的限制（主要受垂直距离的限制）阻碍了高灵敏度端粒酶生物传感器的发展。本工作设计了一种基于螺旋DNA四面体TS引物的电化学端粒酶生物传感器。通过在DNA四面体的顶端添加一个刚性的dsDNA螺旋，我们成功地调节了TS引物与表面之间的距离，从而极大地促进了端粒酶在表面的延伸。信噪比比无螺旋结构的TSP高2倍。计算出的检测限低于10个HeLa细胞，这比其他基于表面延伸的电化学端粒酶传感器低至少2个数量级。STTS传感器的实用性也通过分析包括癌细胞、高端粒酶活性的干细胞和低端粒酶活性的体细胞在内的各种其他细胞系来证明。
The development of sensitive telomerase biosensors is hindered by the restricted accessibility of telomere strand (TS) primer and the limited enzyme reaction space, which is mainly confined by the vertical distance. In this work, we designed an electrochemical telomerase biosensor based on a spired DNA tetrahedron TS primer (STTS). By adding a rigid dsDNA spire onto the top of the DNA tetrahedron, we successfully regulated the distance between the TS primer and the surface, and thus greatly facilitated the telomerase elongation on surface. The signal-to-noise ratio was 2 times higher than TSP without the spire structure. The limit of detection was calculated to be lower than 10 HeLa cells, which is at least 2 magnitudes lower than other surface extension-based electrochemical telomerase sensors without amplification. The practicability of STTS sensor was also demonstrated by analysing various other cell lines including cancer cells, stem cells of high telomerase activity and somatic cells of low telomerase activity.
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影响因子：
15
作者：
Zheng G;Daniel WL;Mirkin CA
通讯作者：
Mirkin CA
影响因子：
12.6
作者：
Zi Yi;Hai-bo Wang;Ke Chen;Qing Gao;Hao Tang;R. Yu;X. Chu
通讯作者：
Zi Yi;Hai-bo Wang;Ke Chen;Qing Gao;Hao Tang;R. Yu;X. Chu
影响因子：
5.3
作者：
LEE, MS;BLACKBURN, EH
通讯作者：
BLACKBURN, EH
影响因子：
12.6
作者：
Li, Ying;Liu, Bangwei;Wei, Qingli
通讯作者：
Wei, Qingli
影响因子：
5.3
作者：
M. Lee;E. Blackburn
通讯作者：
M. Lee;E. Blackburn