A new approach to amplified telomerase detection with polyvalent oligonucleotide nanoparticle conjugates.
A new approach to amplified telomerase detection with polyvalent oligonucleotide nanoparticle conjugates.
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DOI：
10.1021/ja803035p
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发表时间：
2008-07-30
影响因子：
15
通讯作者：
Mirkin CA
中科院分区：
文献类型：
--
作者：
Zheng G;Daniel WL;Mirkin CA
我们报道了一种新的检测人类端粒酶活性的方法，该方法依赖于多价寡核苷酸纳米颗粒偶联物作为诊断探针和扩增单元。用特定的寡核苷酸序列功能化的金纳米颗粒可以有效地捕获端粒酶，并随后被延长。同时测量延长的和未修饰的寡核苷酸序列。这两条链不仅相互作为内部阳性对照，而且还提供了一种放大信号的方法。在高浓度下，伸长的和未修饰的链都表现出可测量的响应。在低端粒酶浓度（例如，来自10个HeLa细胞），不能检测到伸长的链，但是可以检测到来自相同探针颗粒的未修饰的序列，因为它们的浓度更高，提供了一种新的扩增形式。这种新的检测方法的灵敏度可以与传统的基于PCR的端粒酶检测方法相媲美。
We report a new assay for human telomerase activity that relies on polyvalent oligonucleotide nanoparticle conjugates as diagnostic probes and amplification units. Gold nanoparticles functionalized with specific oligonucleotide sequences can efficiently capture telomerase enzymes and subsequently be elongated. Both the elongated and unmodified oligonucleotide sequences are simultaneously measured. The two strands not only serve as internal positive controls for each other but also provide a way of amplifying signal. At high concentrations, both elongated and unmodified strands exhibit measurable responses. At low telomerase concentrations (e.g., from 10 HeLa cells), elongated strands cannot be detected, but the unmodified sequences, which come from the same probe particles, can be detected because their concentration is higher, providing a novel form of amplification. This new assay rivals the sensitivity of the conventional PCR-based method of telomerase detection.
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影响因子：
64.8
作者：
Mirkin, CA;Letsinger, RL;Storhoff, JJ
通讯作者：
Storhoff, JJ
影响因子：
15
作者：
Liu, J;Lu, Y
通讯作者：
Lu, Y
影响因子：
15
作者：
Weizmann, Y;Patolsky, F;Willner, I
通讯作者：
Willner, I
影响因子：
56.9
作者：
FENG, JL;FUNK, WD;VILLEPONTEAU, B
通讯作者：
VILLEPONTEAU, B
影响因子：
15
作者：
Peña, SRN;Raina, S;Keating, CD
通讯作者：
Keating, CD