Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.
Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.
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海洋来源的三阳链霉菌 FMA 吲哚并咔唑生物合成基因簇的克隆、表征和异源表达
DOI：
10.3390/md11020466
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发表时间：
2013-02-06
期刊：
影响因子：
5.4
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Li T;Du Y;Cui Q;Zhang J;Zhu W;Hong K;Li W
吲哚咔唑（ICZ）生物碱因其独特的结构和潜在的治疗应用而备受关注。从一株海洋源放线菌三洋链霉菌FMA中分离鉴定了一系列icz。为了阐明三叶草FMA中ICZ产生的生物合成机制，利用退化引物进行PCR克隆了ICZ环形成的fad依赖单加氧酶基因片段，并以该片段为探针分离出含有spc基因簇的34.6 kb DNA区域。序列分析显示ICZ环形成（spcO， D， P， C）、糖单元形成（spcA， B， E， K， J， I）、糖基化（spcN， G）、甲基化（spcMA， MB）和调控（spcR）基因。通过基因失活和异源表达在冷色链霉菌M1152中证实了它们参与ICZ的生物合成。本研究首次从海洋源放线菌中克隆并鉴定了ICZ基因簇，为深入了解ICZ糖苷的生物合成机制奠定了基础。
The indolocarbazole (ICZ) alkaloids have attracted much attention due to their unique structures and potential therapeutic applications. A series of ICZs were recently isolated and identified from a marine-derived actinomycete strain, Streptomyces sanyensis FMA. To elucidate the biosynthetic machinery associated with ICZs production in S. sanyensis FMA, PCR using degenerate primers was carried out to clone the FAD-dependent monooxygenase gene fragment for ICZ ring formation, which was used as a probe to isolate the 34.6-kb DNA region containing the spc gene cluster. Sequence analysis revealed genes for ICZ ring formation (spcO, D, P, C), sugar unit formation (spcA, B, E, K, J, I), glycosylation (spcN, G), methylation (spcMA, MB), as well as regulation (spcR). Their involvement in ICZ biosynthesis was confirmed by gene inactivation and heterologous expression in Streptomyces coelicolor M1152. This work represents the first cloning and characterization of an ICZ gene cluster isolated from a marine-derived actinomycete strain and would be helpful for thoroughly understanding the biosynthetic mechanism of ICZ glycosides.
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影响因子：
2.1
作者：
Ishikawa, J;Hotta, K
通讯作者：
Hotta, K
影响因子：
6.7
作者：
Acero, Nuria;Brana, Miguel F.;Piulats, Jaume
通讯作者：
Piulats, Jaume
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.3
作者：
Hernández, LMC;Blanco, JAD;Grávalos, DG
通讯作者：
Grávalos, DG