Synergistic interplay between the two major bone minerals, hydroxyapatite and whitlockite nanoparticles, for osteogenic differentiation of mesenchymal stem cells.
Synergistic interplay between the two major bone minerals, hydroxyapatite and whitlockite nanoparticles, for osteogenic differentiation of mesenchymal stem cells.
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DOI：
10.1016/j.actbio.2018.01.016
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发表时间：
2018-03-15
影响因子：
9.7
通讯作者：
Jang HL
中科院分区：
文献类型：
--
作者：
Cheng H;Chabok R;Guan X;Chawla A;Li Y;Khademhosseini A;Jang HL
人骨的无机部分主要由羟基磷灰石（HAP：Ca 10（PO 4）6（OH）2）和白磷钙石（WH：Ca 18 Mg 2（HPO 4）2（PO 4）12）矿物组成，其中WH相占总重量的20-35%。这两种骨矿物质具有不同的晶体结构和物理化学性质，这意味着它们在骨生理学中的独特作用。然而，到目前为止，骨骼中HAP和WH之间存在一定比例的生物学意义尚不清楚。为了解决这一基本问题，骨模拟支架被设计成包封人间充质干细胞（MSC），用于根据不同比例的HAP和WH评估其成骨活性。有趣的是，当MSC以3：1的HAP和WH纳米颗粒比例生长时，细胞生长和成骨分化显著促进，这与骨相似。水凝胶支架中HAP和WH之间的这种协同作用的原因之一是，虽然与HAP相比，WH纳米颗粒可以增强MSC的成骨分化，但WH违反直觉地降低了纳米复合水凝胶的机械刚度并阻碍了细胞的成骨活性。总之，这些发现确定了骨模拟支架中两种主要矿物质之间的最佳比例，以最大限度地提高MSC的成骨分化。
The inorganic part of human bone is mainly composed of hydroxyapatite (HAP: Ca10(PO4)6(OH)2) and whitlockite (WH: Ca18Mg2(HPO4)2(PO4)12) minerals, where the WH phase occupies up to 20-35% of total weight. These two bone minerals have different crystal structures and physicochemical properties, implying their distinguished role in bone physiology. However, until now, the biological significance of the presence of a certain ratio between HAP and WH in bone is unclear. To address this fundamental question, bone mimetic scaffolds are designed to encapsulate human mesenchymal stem cells (MSCs) for assessing their osteogenic activity depending on different ratios of HAP and WH. Interestingly, cellular growth and osteogenic differentiation are significantly promoted when MSCs are grown with a 3 to 1 ratio of HAP and WH nanoparticles, which is similar to bone. One of the reasons for this synergism between HAP and WH in hydrogel scaffolds is that, while WH nanoparticles can enhance osteogenic differentiation of MSCs compared to HAP, WH counterintuitively decreases the mechanical stiffness of nanocomposite hydrogels and hinders the osteogenic activity of cells. Taken together, these findings identify the optimal ratio between two major minerals in bone mimetic scaffolds to maximize the osteogenic differentiation of MSCs.
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影响因子：
1.8
作者：
Kim, Ho Jin;Lee, Mi Jin;Kim, Jang Eok
通讯作者：
Kim, Jang Eok
影响因子：
--
作者：
Kadiyala, S;Jaiswal, N;Bruder, SP
通讯作者：
Bruder, SP
影响因子：
17.1
作者：
Jang, Hae Lin;Jin, Kyoungsuk;Nam, Ki Tae
通讯作者：
Nam, Ki Tae
DOI：
10.3181/00379727-105-26111
发表时间：
1960-01-01
影响因子：
--
作者：
BREIBART, S;LEE, JS;FORBES, GB
通讯作者：
FORBES, GB
DOI：
10.2106/00004623-200310000-00010
发表时间：
2003-10-01
影响因子：
5.3
作者：
Arinzeh, TL;Peter, SJ;Kadiyala, S
通讯作者：
Kadiyala, S