Quantitative analysis of SARS-CoV-2 RNA from wastewater solids in communities with low COVID-19 incidence and prevalence.
Quantitative analysis of SARS-CoV-2 RNA from wastewater solids in communities with low COVID-19 incidence and prevalence.
复制标题
DOI：
10.1016/j.watres.2020.116560
复制
发表时间：
2021-01-01
期刊：
影响因子：
12.8
通讯作者：
Delatolla R
中科院分区：
文献类型：
--
作者：
D'Aoust PM;Mercier E;Montpetit D;Jia JJ;Alexandrov I;Neault N;Baig AT;Mayne J;Zhang X;Alain T;Langlois MA;Servos MR;MacKenzie M;Figeys D;MacKenzie AE;Graber TE;Delatolla R
在缺乏预防 COVID-19 的有效疫苗的情况下，重要的是能够跟踪社区感染，为旨在减少传播的公共卫生干预措施提供信息，从而减轻医疗保健压力，改善健康结果并减少经济不确定性。废水监测已迅速成为一种潜在工具，通过测量废水系统中 RNA 信号的趋势来有效监测社区感染。在这项研究中，对从服务于加拿大国家首都地区（即安大略省渥太华市（人口约 1.1M）和魁北克省加蒂诺市（人口约 28 万）的两个水资源回收设施 (WRRF) 的进水后沉砂固体 (PGS) 和初级澄清污泥 (PCS) 收集的固体中收集的固体中的 SARS-CoV-2 病毒 RNA N1 和 N2 基因区域进行了定量。与 RT-qPCR 相比，PCS 样品使用 RT-ddPCR 显示信号抑制，PGS 样品使用两种检测显示相似的可定量 RNA 浓度。与 PGS 样本 (79.2, 82.3%, n = 5) 相比，RT-qPCR 显示 PCS 中 N1 和 N2 基因区域的检测频率更高 (92.7, 90.6%, n = 6)。因此，PCS 采样可能是 SARS-CoV-2 病毒定量的有效方法，特别是在社区 COVID-19 发病率下降和较低的时期。与拟杆菌 16S rRNA 或人类 18S rRNA 相比，无论环境条件如何，胡椒轻度斑驳病毒 (PMMoV) 在三个月的时间里，两次 WRRF 在 PCS 中的 RNA 信号变化较小，这使得 PMMoV 成为 SARS-CoV-2 信号正常化的潜在有用生物标志物。来自两个城市 WRRF 的 PMMoV 标准化 PCS RNA 信号与区域公共卫生流行病学指标相关，确定粪便指标标准化 PCS (PMMoV) 作为监测社区 SARS-Cov-2 感染减少和低发病率期间趋势的潜在有效工具。
In the absence of an effective vaccine to prevent COVID-19 it is important to be able to track community infections to inform public health interventions aimed at reducing the spread and therefore reduce pressures on health-care, improve health outcomes and reduce economic uncertainty. Wastewater surveillance has rapidly emerged as a potential tool to effectively monitor community infections through measuring trends of RNA signal in wastewater systems. In this study SARS-CoV-2 viral RNA N1 and N2 gene regions are quantified in solids collected from influent post grit solids (PGS) and primary clarified sludge (PCS) in two water resource recovery facilities (WRRF) serving Canada's national capital region, i.e., the City of Ottawa, ON (pop. ≈ 1.1M) and the City of Gatineau, QC (pop. ≈ 280K). PCS samples show signal inhibition using RT-ddPCR compared to RT-qPCR, with PGS samples showing similar quantifiable concentrations of RNA using both assays. RT-qPCR shows higher frequency of detection of N1 and N2 gene regions in PCS (92.7, 90.6%, n = 6) as compared to PGS samples (79.2, 82.3%, n = 5). Sampling of PCS may therefore be an effective approach for SARS-CoV-2 viral quantification, especially during periods of declining and low COVID-19 incidence in the community. The pepper mild mottle virus (PMMoV) is determined to have a less variable RNA signal in PCS over a three month period for two WRRFs, regardless of environmental conditions, compared to Bacteroides 16S rRNA or human 18S rRNA, making PMMoV a potentially useful biomarker for normalization of SARS-CoV-2 signal. PMMoV-normalized PCS RNA signal from WRRFs of two cities correlated with the regional public health epidemiological metrics, identifying PCS normalized to a fecal indicator (PMMoV) as a potentially effective tool for monitoring trends during decreasing and low-incidence of infection of SARS-Cov-2 in communities.
登录
查看更多内容
影响因子：
4.4
作者：
Bernhard, AE;Field, KG
通讯作者：
Field, KG
影响因子：
11.4
作者：
Armanious, Antonius;Aeppli, Meret;Sander, Michael
通讯作者：
Sander, Michael
冠状病毒在水和废水中的生存
影响因子：
3.4
作者：
Gundy PM;Gerba CP;Pepper IL
通讯作者：
Pepper IL
影响因子：
9.8
作者：
Haramoto, Eiji;Malla, Bikash;Kitajima, Masaaki
通讯作者：
Kitajima, Masaaki
影响因子：
12.8
作者：
Hamza, Ibrahim Ahmed;Jurzik, Lars;Wilhelm, Michael
通讯作者：
Wilhelm, Michael