Discovery of a Potential Plasma Protein Biomarker Panel for Acute-on-Chronic Liver Failure Induced by Hepatitis B Virus.
Discovery of a Potential Plasma Protein Biomarker Panel for Acute-on-Chronic Liver Failure Induced by Hepatitis B Virus.
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发现乙型肝炎病毒引起的慢性急性肝衰竭的潜在血浆蛋白生物标志物组
DOI：
10.3389/fphys.2017.01009
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发表时间：
2017
影响因子：
4
通讯作者：
Zhu J
中科院分区：
文献类型：
--
作者：
Zhou N;Wang K;Fang S;Zhao X;Huang T;Chen H;Yan F;Tang Y;Zhou H;Zhu J
B型肝炎病毒（HBV）相关慢性加急性肝功能衰竭（HBV-ACLF）是慢性B型肝炎（CH B）患者肝功能急性恶化的主要表现，目前尚无可预测其预后的生物标志物。血浆是生物标志物发现的理想样品，这是由于廉价和微创采样以及良好的再现性。在这项研究中，高丰度血浆蛋白的免疫耗竭，然后基于iTRAQ的定量蛋白质组学方法，分别分析了20名健康对照者，20名CHB患者和20名HBV-ACLF患者的血浆样本。结果，从这些样品中总共鉴定出427种蛋白质，并且与CHB患者和健康对照相比，HBV-ACLF患者中有42种蛋白质差异表达。根据生物信息学分析结果，选择与免疫应答（MMR）、炎症应答（OPN、HPX）、凝血（ATIII）和脂质代谢（APO-CII、GP 73）相关的6种蛋白作为生物标志物候选物。进一步ELISA分析证实HBV-ACLF血浆样品中GP 73、MMR、OPN显著上调，ATIII、HPX、APO-CII显著下调（p < 0.01）。此外，受试者工作特征（ROC）曲线分析显示这些候选人在评估HBV-ACLF中具有较高的诊断价值。总之，本定量蛋白质组学研究确定了6个新的HBV-ACLF生物标志物候选者，并可能为HBV-ACLF生物标志物的开发提供基础信息。
Hepatitis B virus (HBV)-associated acute-on-chronic liver failure (HBV-ACLF), characterized by an acute deterioration of liver function in the patients with chronic hepatitis B (CHB), is lack of predicting biomarkers for prognosis. Plasma is an ideal sample for biomarker discovery due to inexpensive and minimally invasive sampling and good reproducibility. In this study, immuno-depletion of high-abundance plasma proteins followed by iTRAQ-based quantitative proteomic approach was employed to analyze plasma samples from 20 healthy control people, 20 CHB patients and 20 HBV-ACLF patients, respectively. As a result, a total of 427 proteins were identified from these samples, and 42 proteins were differentially expressed in HBV-ACLF patients as compared to both CHB patients and healthy controls. According to bioinformatics analysis results, 6 proteins related to immune response (MMR), inflammatory response (OPN, HPX), blood coagulation (ATIII) and lipid metabolism (APO-CII, GP73) were selected as biomarker candidates. Further ELISA analysis confirmed the significant up-regulation of GP73, MMR, OPN and down-regulation of ATIII, HPX, APO-CII in HBV-ACLF plasma samples (p < 0.01). Moreover, receiver operating characteristic (ROC) curve analysis revealed high diagnostic value of these candidates in assessing HBV-ACLF. In conclusion, present quantitative proteomic study identified 6 novel HBV-ACLF biomarker candidates and might provide fundamental information for development of HBV-ACLF biomarker.
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DOI：
10.1158/1940-6207.capr-15-0434
发表时间：
2016-09
期刊：
Cancer prevention research (Philadelphia, Pa.)
影响因子：
--
作者：
Duarte-Salles T;Misra S;Stepien M;Plymoth A;Muller D;Overvad K;Olsen A;Tjønneland A;Baglietto L;Severi G;Boutron-Ruault MC;Turzanski-Fortner R;Kaaks R;Boeing H;Aleksandrova K;Trichopoulou A;Lagiou P;Bamia C;Pala V;Palli D;Mattiello A;Tumino R;Naccarati A;Bueno-de-Mesquita HB;Peeters PH;Weiderpass E;Quirós JR;Agudo A;Sánchez-Cantalejo E;Ardanaz E;Gavrila D;Dorronsoro M;Werner M;Hemmingsson O;Ohlsson B;Sjöberg K;Wareham NJ;Khaw KT;Bradbury KE;Gunter MJ;Cross AJ;Riboli E;Jenab M;Hainaut P;Beretta L
通讯作者：
Beretta L
影响因子：
48
作者：
Geiger, Tamar;Cox, Juergen;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
13.5
作者：
KOBAYASHI, J;YAMADA, S;KAWASAKI, H
通讯作者：
KAWASAKI, H
影响因子：
3.4
作者：
He, QY;Lau, GKK;Chiu, JF
通讯作者：
Chiu, JF
DOI：
10.1111/j.1440-1746.1997.tb00351.x
发表时间：
1997-01-01
影响因子：
4.1
作者：
Castelino, DJ;Salem, HH
通讯作者：
Salem, HH