Impact of spatial organization on a novel auxotrophic interaction among soil microbes.
Impact of spatial organization on a novel auxotrophic interaction among soil microbes.
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DOI：
10.1038/s41396-018-0095-z
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Soyer OS
中科院分区：
文献类型：
--
作者：
Jiang X;Zerfaß C;Feng S;Eichmann R;Asally M;Schäfer P;Soyer OS
深入了解微生物群落和设计合成微生物群落的一个关键前提是确定关键物种之间的个体代谢相互作用以及这些相互作用如何受到不同环境因素的影响。在空间组织的环境中，破译种-种和种-环境相互作用的生理基础，需要还原论的方法，使用生态和功能相关的物种。为此，我们在这里集中在一个定义的系统来研究植物有益的内生真菌Serendipita indica和土壤中的模式细菌枯草芽孢杆菌之间的空间背景下的代谢相互作用。重点研究了S. indica在规定的条件下，我们确定了一个营养缺陷型，在这种生物体的硫胺素，这是一个关键的辅因子的基本反应，在中央碳代谢。我们发现S.当与B共培养时，在不含硫胺素的培养基中恢复了籼稻生长。枯草芽孢杆菌然而，这种营养缺陷型相互作用的成功依赖于系统的空间和时间组织; B的有益影响。枯草芽孢杆菌只有在与S.无论是在时间还是空间上。这些研究结果描述了一个关键的营养缺陷型的相互作用，在土壤中的有机体之间的植物生态系统的功能是重要的，并指出了潜在的重要性，空间和时间组织的营养缺陷型的相互作用的成功。这些点对于作为植物种子处理的最小功能合成群落的工程设计和在限定条件下的垂直耕作特别重要。
A key prerequisite to achieve a deeper understanding of microbial communities and to engineer synthetic ones is to identify the individual metabolic interactions among key species and how these interactions are affected by different environmental factors. Deciphering the physiological basis of species–species and species–environment interactions in spatially organized environments requires reductionist approaches using ecologically and functionally relevant species. To this end, we focus here on a defined system to study the metabolic interactions in a spatial context among the plant-beneficial endophytic fungus Serendipita indica, and the soil-dwelling model bacterium Bacillus subtilis. Focusing on the growth dynamics of S. indica under defined conditions, we identified an auxotrophy in this organism for thiamine, which is a key co-factor for essential reactions in the central carbon metabolism. We found that S. indica growth is restored in thiamine-free media, when co-cultured with B. subtilis. The success of this auxotrophic interaction, however, was dependent on the spatial and temporal organization of the system; the beneficial impact of B. subtilis was only visible when its inoculation was separated from that of S. indica either in time or space. These findings describe a key auxotrophic interaction in the soil among organisms that are shown to be important for plant ecosystem functioning, and point to the potential importance of spatial and temporal organization for the success of auxotrophic interactions. These points can be particularly important for engineering of minimal functional synthetic communities as plant seed treatments and for vertical farming under defined conditions.
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影响因子：
64.8
作者：
Chatterjee, Abhishek;Abeydeera, N. Dinuka;Bale, Shridhar;Pai, Pei-Jing;Dorrestein, Pieter C.;Russell, David H.;Ealick, Steven E.;Begley, Tadhg P.
通讯作者：
Begley, Tadhg P.
影响因子：
14.9
作者：
Federhen S
通讯作者：
Federhen S
影响因子：
5.4
作者：
Grosskopf, Tobias;Soyer, Orkun S.
通讯作者：
Soyer, Orkun S.
影响因子：
3.4
作者：
Grosskopf, Tobias;Consuegra, Jessika;Schneider, Dominique
通讯作者：
Schneider, Dominique
影响因子：
4.1
作者：
Gòdia, F;Albiol, J;Lasseur, C
通讯作者：
Lasseur, C