Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63.
Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63.
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DOI：
10.1016/j.cub.2018.05.092
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发表时间：
2018-08-20
期刊：
影响因子：
--
通讯作者：
Dodd AN
中科院分区：
文献类型：
--
作者：
Frank A;Matiolli CC;Viana AJC;Hearn TJ;Kusakina J;Belbin FE;Wells Newman D;Yochikawa A;Cano-Ramirez DL;Chembath A;Cragg-Barber K;Haydon MJ;Hotta CT;Vincentz M;Webb AAR;Dodd AN
生物钟与昼夜周期的同步确保了生物事件的正确计时。这种夹带过程对于确保昼夜节律振荡器的相位与环境中的日常事件同步至关重要，从而能够准确预测环境变化。植物中的夹带需要昼夜节律振荡器通过未识别的途径发生相变，从而改变昼夜节律振荡器基因表达以响应光、温度和糖。为了确定生物钟如何响应代谢节律，我们研究了拟南芥中糖调节昼夜节律阶段的机制。我们关注代谢调节，因为在几个实验系统中昼夜节律振荡器和代谢之间会发生相互作用，但分子机制尚不清楚。在这里，我们证明转录因子 BASIC LEUCINE ZIPPER63 (bZIP63) 调节昼夜节律振荡器基因 PSEUDO RESPONSE REGULATOR7 (PRR7) 以改变昼夜节律阶段以响应糖。我们发现 SnRK1（一种调节 bZIP63 活性和昼夜节律的糖感应激酶）是蔗糖诱导的昼夜节律变化所必需的。此外，TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1) 可合成信号糖海藻糖-6-磷酸，是响应蔗糖的昼夜节律相位调整所必需的。我们证明，能量可用性的每日节律可以通过 bZIP63、TPS1 和 SnRK1 能量传感器的 KIN10 亚基的功能来带动昼夜节律振荡器。这确定了一种根据糖来调整昼夜节律阶段的分子机制。转录因子 bZIP63 结合并调节生物钟基因 PRR7 bZIP63 是通过糖海藻糖-6-磷酸代谢和 KIN10 信号调节昼夜节律周期进行调节所必需的 糖信号在光和暗周期中建立正确的昼夜节律阶段 Frank 等人。确定拟南芥生物钟夹带糖的机制。昼夜节律阶段的代谢调节涉及海藻糖-6-磷酸、SnRK1 亚基 KIN10 和转录因子 bZIP63。 bZIP63 调节生物钟基因 PSEUDORESPONSE REGULATOR7。这设置了光明和黑暗周期的昼夜节律阶段。
Synchronization of circadian clocks to the day-night cycle ensures the correct timing of biological events. This entrainment process is essential to ensure that the phase of the circadian oscillator is synchronized with daily events within the environment, to permit accurate anticipation of environmental changes. Entrainment in plants requires phase changes in the circadian oscillator, through unidentified pathways, which alter circadian oscillator gene expression in response to light, temperature, and sugars. To determine how circadian clocks respond to metabolic rhythms, we investigated the mechanisms by which sugars adjust the circadian phase in Arabidopsis. We focused upon metabolic regulation because interactions occur between circadian oscillators and metabolism in several experimental systems, but the molecular mechanisms are unidentified. Here, we demonstrate that the transcription factor BASIC LEUCINE ZIPPER63 (bZIP63) regulates the circadian oscillator gene PSEUDO RESPONSE REGULATOR7 (PRR7) to change the circadian phase in response to sugars. We find that SnRK1, a sugar-sensing kinase that regulates bZIP63 activity and circadian period is required for sucrose-induced changes in circadian phase. Furthermore, TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1), which synthesizes the signaling sugar trehalose-6-phosphate, is required for circadian phase adjustment in response to sucrose. We demonstrate that daily rhythms of energy availability can entrain the circadian oscillator through the function of bZIP63, TPS1, and the KIN10 subunit of the SnRK1 energy sensor. This identifies a molecular mechanism that adjusts the circadian phase in response to sugars. The transcription factor bZIP63 binds and regulates the circadian clock gene PRR7 bZIP63 is required for adjustment of circadian period by sugars Trehalose-6-phosphate metabolism and KIN10 signaling regulate circadian period Sugar signals establish the correct circadian phase in light and dark cycles Frank et al. identify mechanisms by which the Arabidopsis circadian clock entrains to sugars. Metabolic adjustment of circadian phase involves trehalose-6-phosphate, SnRK1 subunit KIN10 and the transcription factor bZIP63. bZIP63 regulates the circadian clock gene PSEUDORESPONSE REGULATOR7. This sets the circadian phase in light and dark cycles.
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影响因子：
4.5
作者：
Kubota A;Ito S;Shim JS;Johnson RS;Song YH;Breton G;Goralogia GS;Kwon MS;Laboy Cintrón D;Koyama T;Ohme-Takagi M;Pruneda-Paz JL;Kay SA;MacCoss MJ;Imaizumi T
通讯作者：
Imaizumi T
影响因子：
56.9
作者：
Dodd, Antony N.;Gardner, Michael J.;Webb, Alex A. R.
通讯作者：
Webb, Alex A. R.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
7.2
作者：
Ehlert, Andrea;Weltmeier, Fridtjof;Droege-Laser, Wolfgang
通讯作者：
Droege-Laser, Wolfgang
影响因子：
11.6
作者：
Gould, PD;Locke, JCW;Hall, A
通讯作者：
Hall, A