CsrA impacts survival of Yersinia enterocolitica by affecting a myriad of physiological activities.
CsrA impacts survival of Yersinia enterocolitica by affecting a myriad of physiological activities.
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DOI：
10.1186/s12866-015-0343-6
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发表时间：
2015-02-14
期刊：
影响因子：
4.2
通讯作者：
Young GM
中科院分区：
文献类型：
--
作者：
LeGrand K;Petersen S;Zheng Y;Liu KK;Ozturk G;Chen JY;Young GM
先前的一项研究发现了小肠结肠炎耶尔森氏菌转座子突变体 GY448，该突变体无法输出鞭毛三型分泌系统 (T3SS) 依赖性磷脂酶 YplA。该菌株也缺乏运动性，无法在 Lauria-Bertani 琼脂培养基上形成菌落。初步分析表明其携带csrA突变。大肠杆菌中的 CsrA 是一种 RNA 结合蛋白，参与多种生理活动的特异性转录后调节。本研究调查了 CsrA 如何影响控制 YplA 输出和运动的鞭毛调控级联的表达。它还探讨了 csrA 突变对小肠结肠炎耶尔森氏菌的影响，以响应对自然和实验室环境中生长重要的生理变化的条件。 GMY448 中转座子插入的精确位置被映射到 csrA 内。基因互补恢复了运动性和 YplA 输出表型 (Yex) 的破坏，这证实了这种突变破坏了 CsrA 功能。 csrA 的突变通过改变主调节因子 flhDC 的表达来影响 yplA 和鞭毛基因的表达，这些基因参与鞭毛 T3SS 依赖性输出和运动。 csrA 的突变还导致小肠结肠炎耶尔森氏菌对各种渗透剂、温度和抗生素的敏感性增加。这项研究的结果揭示了 CsrA 在小肠结肠炎耶尔森氏菌中如何发挥作用来控制其生理机能的独特方面。这提供了关于 Csr 系统如何容易适应特定环境和细菌生活方式的观点。
A previous study identified a Yersinia enterocolitica transposon mutant, GY448, that was unable to export the flagellar type three secretion system (T3SS)-dependent phospholipase, YplA. This strain was also deficient for motility and unable to form colonies on Lauria-Bertani agar medium. Preliminary analysis suggested it carried a mutation in csrA. CsrA in Escherichia coli is an RNA-binding protein that is involved in specific post-transcriptional regulation of a myriad of physiological activities. This study investigated how CsrA affects expression of the flagellar regulatory cascade that controls YplA export and motility. It also explored the effect of csrA mutation on Y. enterocolitica in response to conditions that cue physiological changes important for growth in environments found both in nature and the laboratory. The precise location of the transposon insertion in GMY448 was mapped within csrA. Genetic complementation restored disruptions in motility and the YplA export phenotype (Yex), which confirmed this mutation disrupted CsrA function. Mutation of csrA affected expression of yplA and flagellar genes involved in flagellar T3SS dependent export and motility by altering expression of the master regulators flhDC. Mutation of csrA also resulted in increased sensitivity of Y. enterocolitica to various osmolytes, temperatures and antibiotics. The results of this study reveal unique aspects of how CsrA functions in Y. enterocolitica to control its physiology. This provides perspective on how the Csr system is susceptible to adaptation to particular environments and bacterial lifestyles.
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影响因子：
5.1
作者：
Jonas K;Edwards AN;Ahmad I;Romeo T;Römling U;Melefors O
通讯作者：
Melefors O
影响因子：
3.2
作者：
IRIARTE, M;STAINIER, I;CORNELIS, GR
通讯作者：
CORNELIS, GR
影响因子：
3.6
作者：
Kapatral, V;Olson, JW;Minnich, SA
通讯作者：
Minnich, SA
影响因子：
4.8
作者：
Liu, MY;Gui, GJ;Romeo, T
通讯作者：
Romeo, T
影响因子：
3.5
作者：
Chatterjee, Asita;Cui, Yaya;Chatterjee, Arun K.
通讯作者：
Chatterjee, Arun K.