Phosphonated trityl probes for concurrent in vivo tissue oxygen and pH monitoring using electron paramagnetic resonance-based techniques.
Phosphonated trityl probes for concurrent in vivo tissue oxygen and pH monitoring using electron paramagnetic resonance-based techniques.
复制标题
DOI：
10.1021/ja401572r
复制
发表时间：
2013-04-17
影响因子：
15
通讯作者：
Khramtsov, Valery V.
中科院分区：
文献类型：
--
作者：
Dhimitruka, Ilirian;Bobko, Andrey A.;Eubank, Timothy D.;Komarov, Denis A.;Khramtsov, Valery V.
Previously we proposed the concept of dual function pH and oxygen paramagnetic probes based on the incorporation of ionizable groups into the structure of persistent triarylmethyl radicals, TAMs (J. Am. Chem. Soc. 2007, 129, 7240–7241). In this paper, we synthesized an asymmetric monophosphonated TAM probe with the simplest doublet hfs pattern ideally suited for dual function electron paramagnetic resonance (EPR)-based applications. An extraordinary low line width of the synthesized deuterated derivative, p1TAM-D (ΔHpp ≤ 50 mG, Lorentz line width, ≤20 mG) results in high sensitivity to pO2 due to oxygen-induced line broadening (ΔLW/ΔpO2 ≈ 0.5 mG/mmHg or ≈400 mG/mM); accuracy of pO2 measurement, ≈1 mmHg). The presence of a phosphono group in the p1TAM-D structure provides pH sensitivity to its EPR spectra in the physiological range of pH from 5.9 to 8.2 with the ratio of signal intensities of protonated and deprotonated states being a reliable pH marker (accuracy of pH measurements, ± 0.05). The independent character of pH and [O2] effects on the EPR spectra of p1TAM-D provides dual functionality to this probe. The L-band EPR studies performed in breast tumor-bearing mice show a significant difference in extracellular pH and pO2 between tumor and normal mammary gland tissues, as well as the effect of animal breathing with 100% O2 on tissue oxygenation. The developed dual function phosphonated p1TAM-D probe provides a unique tool for in vivo concurrent tissue oxygen and pH monitoring.
登录
查看更多内容
影响因子：
3.4
作者：
Presley, Tennille;Kuppusamy, Periannan;Ilangovan, Govindasamy
通讯作者：
Ilangovan, Govindasamy
影响因子：
14
作者：
Mader, K;Gallez, B;Swartz, HM
通讯作者：
Swartz, HM
双功能pH和氧磷酸trityl探针。
影响因子：
7.4
作者：
Bobko, Andrey A.;Dhimitruka, Ilirian;Komarov, Denis A.;Khramtsov, Valery V.
通讯作者：
Khramtsov, Valery V.
DOI：
10.1073/pnas.042671399
发表时间：
2002-02-19
影响因子：
11.1
作者：
Krishna, MC;English, S;Mitchell, JB
通讯作者：
Mitchell, JB
影响因子：
3.8
作者：
Epel, Boris;Sundramoorthy, Subramanian V.;Halpern, Howard J.
通讯作者：
Halpern, Howard J.