Induction of input-specific spine shrinkage on dendrites of rodent hippocampal CA1 neurons using two-photon glutamate uncaging.
Induction of input-specific spine shrinkage on dendrites of rodent hippocampal CA1 neurons using two-photon glutamate uncaging.
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DOI：
10.1016/j.xpro.2021.100996
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Zito K
中科院分区：
文献类型：
--
作者：
Jang J;Anisimova M;Oh WC;Zito K
树突棘的萎缩和缺失是支持学习的神经元可塑性的重要组成部分。为了研究脊柱收缩和丢失的机制，Oh及其同事建立了一种双光子谷氨酸释放方案，该方案可靠地诱导啮齿动物海马CA1锥体神经元树突上的输入特异性脊柱收缩。在这里，我们提供了该协议的详细描述，也可以用来诱导输入和突触特异性收缩的树突棘在生理Ca2+水平的优化版本。有关本方案使用和执行的完整详细信息，请参见、和。笼状谷氨酸的双光子光解诱导树突棘收缩使用延时双光子显微镜对稀疏标记神经元的实时成像使用荧光测量对树突棘大小的定量分析器官型海马切片培养物的制备和转染树突棘的收缩和丢失是支持学习的神经元可塑性的重要组成部分。为了研究脊柱收缩和丢失的机制，Oh及其同事建立了一种双光子谷氨酸释放方案，该方案可靠地诱导啮齿动物海马CA1锥体神经元树突上的输入特异性脊柱收缩。在这里，我们提供了该协议的详细描述，也可以用来诱导输入和突触特异性收缩的树突棘在生理Ca2+水平的优化版本。
Shrinkage and loss of dendritic spines are vital components of the neuronal plasticity that supports learning. To investigate the mechanisms of spine shrinkage and loss, Oh and colleagues established a two-photon glutamate uncaging protocol that reliably induces input-specific spine shrinkage on dendrites of rodent hippocampal CA1 pyramidal neurons. Here, we provide a detailed description of that protocol and also an optimized version that can be used to induce input- and synapse-specific shrinkage of dendritic spines at physiological Ca2+ levels. For complete details on the use and execution of this protocol, please refer to, and. Two-photon photolysis of caged glutamate to induce dendritic spine shrinkage Live imaging of sparsely labeled neurons using time-lapse two-photon microscopy Quantitative analysis of dendritic spine size using fluorescence measurements Preparation and transfection of organotypic hippocampal slice cultures Shrinkage and loss of dendritic spines are vital components of the neuronal plasticity that supports learning. To investigate the mechanisms of spine shrinkage and loss, Oh and colleagues established a two-photon glutamate uncaging protocol that reliably induces input-specific spine shrinkage on dendrites of rodent hippocampal CA1 pyramidal neurons. Here, we provide a detailed description of that protocol and also an optimized version that can be used to induce input- and synapse-specific shrinkage of dendritic spines at physiological Ca2+ levels.
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影响因子：
8.8
作者：
Stein IS;Park DK;Claiborne N;Zito K
通讯作者：
Zito K
影响因子：
1.2
作者：
Opitz-Araya, Ximena;Barria, Andres
通讯作者：
Barria, Andres
影响因子：
16.2
作者：
Holtmaat, AJGD;Trachtenberg, JT;Svoboda, K
通讯作者：
Svoboda, K
DOI：
10.3791/675
发表时间：
2008-02-13
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Woods, Georgia;Zito, Karen
通讯作者：
Zito, Karen
影响因子：
5.3
作者：
Stein, Ivar S.;Gray, John A.;Zito, Karen
通讯作者：
Zito, Karen