Differential susceptibility of C57BL/6NCr and B6.Cg-Ptprca mice to commensal bacteria after whole body irradiation in translational bone marrow transplant studies.
Differential susceptibility of C57BL/6NCr and B6.Cg-Ptprca mice to commensal bacteria after whole body irradiation in translational bone marrow transplant studies.
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DOI：
10.1186/1479-5876-6-10
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发表时间：
2008-02-28
影响因子：
7.4
通讯作者：
Wilkinson JE
中科院分区：
文献类型：
--
作者：
Duran-Struuck R;Hartigan A;Clouthier SG;Dyson MC;Lowler K;Gatza E;Tawara I;Toubai T;Weisiger E;Hugunin K;Reddy P;Wilkinson JE
小鼠是骨髓移植（BMT）转化研究的重要且广泛使用的动物模型。在这里，我们记录了一个意外的增加，在同类小鼠进行骨髓移植的死亡率的过程。使用对数秩检验分析35个BMT的生存差异。通过体格检查、尸检、组织病理学、感染性疾病抗体血清学和细菌培养评价受累动物。严重菌血症被确定为主要死亡原因。组织病理学观察到胃肠道（GI）损害。菌血症最可能是由胃肠道细菌移位和清髓性照射引起的免疫抑制引起的。受影响的动物群体的变异性是由伽马和X射线辐射水平的增加以及研究中使用的两种基因几乎相同的小鼠品系的不同敏感性造成的。我们对在三个不同实验室进行的35个鼠BMT的回顾性分析，鉴定出C57 BL/6 NCr（Ly5.1）比B6.Cg-Ptprca/NCr（Ly5.2）更辐射敏感。这是第一份报告，记录了可测量的差异，辐射敏感性和其影响之间的近交系小鼠和其同类对应最终屈服于败血症后骨髓移植。
The mouse is an important and widely utilized animal model for bone marrow transplant (BMT) translational studies. Here, we document the course of an unexpected increase in mortality of congenic mice that underwent BMT. Thirty five BMTs were analyzed for survival differences utilizing the Log Rank test. Affected animals were evaluated by physical examination, necropsy, histopathology, serology for antibodies to infectious disease, and bacterial cultures. Severe bacteremia was identified as the main cause of death. Gastrointestinal (GI) damage was observed in histopathology. The bacteremia was most likely caused by the translocation of bacteria from the GI tract and immunosuppression caused by the myeloablative irradiation. Variability in groups of animals affected was caused by increased levels of gamma and X-ray radiation and the differing sensitivity of the two nearly genetically identical mouse strains used in the studies. Our retrospective analysis of thirty five murine BMTs performed in three different laboratories, identified C57BL/6NCr (Ly5.1) as being more radiation sensitive than B6.Cg-Ptprca/NCr (Ly5.2). This is the first report documenting a measurable difference in radiation sensitivity and its effects between an inbred strain of mice and its congenic counterpart eventually succumbing to sepsis after BMT.
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DOI：
10.1016/j.ijrobp.2004.12.027
发表时间：
2005-07-15
影响因子：
7
作者：
De Ruyck, K;Van Eijkeren, M;Thierens, H
通讯作者：
Thierens, H
影响因子：
2.6
作者：
Lindsay, K. J.;Coates, P. J.;Wright, E. G.
通讯作者：
Wright, E. G.
影响因子：
3.4
作者：
HANSON, WR;FRY, RJM;AINSWORTH, EJ
通讯作者：
AINSWORTH, EJ
影响因子：
6.4
作者：
BROWNSTEIN, DG
通讯作者：
BROWNSTEIN, DG
影响因子：
2.4
作者：
Dietrich, HM;Khaschabi, D;Albini, B
通讯作者：
Albini, B