Cargo-free particles divert neutrophil-platelet aggregates to reduce thromboinflammation.
Cargo-free particles divert neutrophil-platelet aggregates to reduce thromboinflammation.
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DOI：
10.1038/s41467-023-37990-z
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发表时间：
2023-04-28
影响因子：
16.6
通讯作者：
Eniola-Adefeso, Omolola
中科院分区：
文献类型：
--
作者：
Banka, Alison L.;Guevara, M. Valentina;Brannon, Emma R.;Nguyen, Nhien Q.;Song, Shuang;Cady, Gillian;Pinsky, David J.;Uhrich, Kathryn E.;Adili, Reheman;Holinstat, Michael;Eniola-Adefeso, Omolola
炎症和血栓形成的结合是许多心血管疾病的标志。在这样的条件下，血小板通过经由白细胞上的PSGL-1和活化血小板上的P-选择素的相互作用形成血小板-白细胞聚集体而被募集到炎症区域，所述P-选择素可以结合到内皮。虽然颗粒药物载体已被用于被动地将白细胞从炎症区域重定向，但这些载体对血栓炎性病症中血小板积聚的下游影响尚未被研究。在这里，我们探讨了聚合物颗粒转移血小板远离发炎的血管在体外和体内的能力。我们发现，非靶向和靶向的微米级聚合物颗粒可以成功地减少血小板粘附到发炎的内皮细胞单层在体外的血流系统和在体内的脂多糖诱导的，全身性炎症小鼠模型。我们的数据代表了开发无载物抗血小板治疗药物的初步工作，特别是针对血栓炎症的条件。血小板-中性粒细胞聚集是血栓炎症的标志。在这里，作者使用无货物颗粒来阻止血小板-中性粒细胞聚集体的血管壁粘附，这可能成为一种有效的血栓炎症治疗，无论疾病的原因。
The combination of inflammation and thrombosis is a hallmark of many cardiovascular diseases. Under such conditions, platelets are recruited to an area of inflammation by forming platelet-leukocyte aggregates via interaction of PSGL-1 on leukocytes and P-selectin on activated platelets, which can bind to the endothelium. While particulate drug carriers have been utilized to passively redirect leukocytes from areas of inflammation, the downstream impact of these carriers on platelet accumulation in thromboinflammatory conditions has yet to be studied. Here, we explore the ability of polymeric particles to divert platelets away from inflamed blood vessels both in vitro and in vivo. We find that untargeted and targeted micron-sized polymeric particles can successfully reduce platelet adhesion to an inflamed endothelial monolayer in vitro in blood flow systems and in vivo in a lipopolysaccharide-induced, systemic inflammation murine model. Our data represent initial work in developing cargo-free, anti-platelet therapeutics specifically for conditions of thromboinflammation. Platelet-neutrophil aggregates are a hallmark of thromboinflamation. Here, the authors use cargo-free particles to block platelet-neutrophil aggregates’ vascular wall adhesion, which could become an effective thromboinflammation therapy, regardless of disease cause.
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影响因子：
4.3
作者：
Czaja, Benjamin;Gutierrez, Mario;Eniola-Adefeso, Omolola
通讯作者：
Eniola-Adefeso, Omolola
影响因子：
10
作者：
Brannon ER;Kelley WJ;Newstead MW;Banka AL;Uhrich KE;O'Connor CE;Standiford TJ;Eniola-Adefeso O
通讯作者：
Eniola-Adefeso O
DOI：
10.1111/jth.14552
发表时间：
2019-10
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1152/ajpheart.00311.2002
发表时间：
2003-02-01
影响因子：
4.8
作者：
Cerwinka, WH;Cooper, D;Granger, DN
通讯作者：
Granger, DN
影响因子：
6.5
作者：
ENGLAND, JM;BAIN, BJ
通讯作者：
BAIN, BJ