Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon).
Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon).
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DOI：
10.1186/s40659-016-0101-8
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发表时间：
2016-11-08
影响因子：
6.7
通讯作者：
Rodríguez-Sánchez IP
中科院分区：
文献类型：
--
作者：
Pérez-Ibave DC;González-Alvarez R;de La Luz Martinez-Fierro M;Ruiz-Ayma G;Luna-Muñoz M;Martínez-De-Villarreal LE;De Lourdes Garza-Rodríguez M;Reséndez-Pérez D;Mohamed-Noriega J;Garza-Guajardo R;Bautista-De-Lucío VM;Mohamed-Noriega K;Barboza-Quintana O;Arámburo-De-La-Hoz C;Barrera-Saldaña HA;Rodríguez-Sánchez IP
嗅觉素样结构域 (OLFML) 存在于至少四个蛋白质家族中，包括 OLFML2A 和 OLFML2B，它们在成年大鼠视网膜细胞中表达。然而，在人类和狒狒中尚未报道过它们的直系同源表达。本研究的目的是调查 OLFML2A 和 OLFML2B 在狒狒 (Papio hamadryas) 和人类眼组织中的表达，作为阐明 OLFML 在眼生理学中功能的关键。通过RT-PCR对这些物种的眼组织中的OLFML2A和OLFML2B cDNA进行检测。对扩增子进行克隆、测序、系统发育分析，并通过免疫荧光测定确认其蛋白质产物。 OLFML2A 和 OLFML2B 转录本存在于人类角膜、晶状体和视网膜以及狒狒角膜、晶状体、虹膜和视网膜中。狒狒 OLFML2A 和 OLFML2B ORF 序列与其人类的直系同源序列具有 96% 的相似性。 OLFML2A和OLFML2B的进化符合纯化选择的假设。系统发育分析显示 OLFML2A 基因具有明确的同源性，而 OLFML2B 基因的同源性尚不清楚。 OLFML2A 和 OLFML2B 在人类和狒狒眼组织中的表达（包括它们的高度相似性）使狒狒成为推断这些蛋白质在眼睛中的生理和/或代谢功能的强大模型。
The olfactomedin-like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. However, no expression of their orthologous has ever been reported in human and baboon. The aim of this study was to investigate the expression of OLFML2A and OLFML2B in ocular tissues of baboons (Papio hamadryas) and humans, as a key to elucidate OLFML function in eye physiology. OLFML2A and OLFML2B cDNA detection in ocular tissues of these species was performed by RT-PCR. The amplicons were cloned and sequenced, phylogenetically analyzed and their proteins products were confirmed by immunofluorescence assays. OLFML2A and OLFML2B transcripts were found in human cornea, lens and retina and in baboon cornea, lens, iris and retina. The baboon OLFML2A and OLFML2B ORF sequences have 96% similarity with their human’s orthologous. OLFML2A and OLFML2B evolution fits the hypothesis of purifying selection. Phylogenetic analysis shows clear orthology in OLFML2A genes, while OLFML2B orthology is not clear. Expression of OLFML2A and OLFML2B in human and baboon ocular tissues, including their high similarity, make the baboon a powerful model to deduce the physiological and/or metabolic function of these proteins in the eye.
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影响因子：
--
作者：
Kim EB;Kim HK;Hyon JY;Wee WR;Shin YJ
通讯作者：
Shin YJ
影响因子：
19.6
作者：
Sistani, Laleh;Rodriguez, Patricia Q.;Patrakka, Jaakko
通讯作者：
Patrakka, Jaakko
DOI：
10.1073/pnas.90.10.4655
发表时间：
1993-05-15
影响因子：
11.1
作者：
YOKOE, H;ANHOLT, RRH
通讯作者：
ANHOLT, RRH
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
DOI：
10.1073/pnas.95.7.3708
发表时间：
1998-03-31
影响因子：
11.1
作者：
Zhang, JZ;Rosenberg, HF;Nei, M
通讯作者：
Nei, M