High-density marker profiling confirms ancestral genomes of Avena species and identifies D-genome chromosomes of hexaploid oat.
High-density marker profiling confirms ancestral genomes of Avena species and identifies D-genome chromosomes of hexaploid oat.
复制标题
高密度标记分析确定了燕麦属物种的祖先基因组，并鉴定出六倍体燕麦的D基因组染色体。
DOI：
10.1007/s00122-016-2762-7
复制
发表时间：
2016-11
影响因子：
5.4
通讯作者：
Tinker, Nicholas A.
中科院分区：
文献类型：
--
作者：
Yan, Honghai;Bekele, Wubishet A.;Wight, Charlene P.;Peng, Yuanying;Langdon, Tim;Latta, Robert G.;Fu, Yong-Bi;Diederichsen, Axel;Howarth, Catherine J.;Jellen, Eric N.;Boyle, Brian;Wei, Yuming;Tinker, Nicholas A.
对27种燕麦进行了基因组分析，确定了祖先群，描绘了D基因组，并确定了六倍体燕麦21条染色体的祖先起源。我们利用高密度遗传标记对燕麦属27种植物的基因组关系进行了研究。使用了两种GBS分析方法：一种基于先前在栽培六倍体燕麦中定位的标签水平单倍型，另一种旨在采样和计数起源于所有被调查物种的标签水平单倍型。定性上，这两种方法对物种集群和共享祖先基因组给出了类似的预测。此外，结果与以前用常规方法获得的该属的系统发育一致，支持了全基因组GBS分析的稳健性。有证据证明，四倍体A.solaris、A.maroccana(=A.Magna)和A.Murphy的最终和明确分类是包含D-+-C基因组，而不是像过去文献中最常见的那样指定的A-+-C基因组。通过对六倍体燕麦共识图谱中21条染色体代表的电子绘制，我们展示了所定位的六倍体衍生单倍型与AC(DC)基因组四倍体与A和C基因组二倍体之间的相对匹配频率如何能够准确地揭示所有六倍体染色体的基因组起源，包括基因组间易位的大致位置。有证据支持AB四倍体中B基因组的继续分类，并证实现有的A基因组二倍体，包括加拿大燕麦，与四倍体和六倍体燕麦的D基因组足够相似，足以被认为是D基因组二倍体。本文的在线版本(doi：10.1007/s00122-016-2762-7)包含补充材料，授权用户可以使用。
Genome analysis of 27 oat species identifies ancestral groups, delineates the D genome, and identifies ancestral origin of 21 mapped chromosomes in hexaploid oat. We investigated genomic relationships among 27 species of the genus Avena using high-density genetic markers revealed by genotyping-by-sequencing (GBS). Two methods of GBS analysis were used: one based on tag-level haplotypes that were previously mapped in cultivated hexaploid oat (A. sativa), and one intended to sample and enumerate tag-level haplotypes originating from all species under investigation. Qualitatively, both methods gave similar predictions regarding the clustering of species and shared ancestral genomes. Furthermore, results were consistent with previous phylogenies of the genus obtained with conventional approaches, supporting the robustness of whole genome GBS analysis. Evidence is presented to justify the final and definitive classification of the tetraploids A. insularis, A. maroccana (=A. magna), and A. murphyi as containing D-plus-C genomes, and not A-plus-C genomes, as is most often specified in past literature. Through electronic painting of the 21 chromosome representations in the hexaploid oat consensus map, we show how the relative frequency of matches between mapped hexaploid-derived haplotypes and AC (DC)-genome tetraploids vs. A- and C-genome diploids can accurately reveal the genome origin of all hexaploid chromosomes, including the approximate positions of inter-genome translocations. Evidence is provided that supports the continued classification of a diverged B genome in AB tetraploids, and it is confirmed that no extant A-genome diploids, including A. canariensis, are similar enough to the D genome of tetraploid and hexaploid oat to warrant consideration as a D-genome diploid. The online version of this article (doi:10.1007/s00122-016-2762-7) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.1139/b85-144
发表时间：
1985-01-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
BAUM, BR;FEDAK, G
通讯作者：
FEDAK, G
影响因子：
1.9
作者：
Aung, Taing;Zwer, Pamela;Dundas, Ian
通讯作者：
Dundas, Ian
影响因子：
4.1
作者：
DaCosta, Jeffrey M.;Sorenson, Michael D.
通讯作者：
Sorenson, Michael D.
影响因子：
3.7
作者：
Huang YF;Poland JA;Wight CP;Jackson EW;Tinker NA
通讯作者：
Tinker NA
影响因子：
3.1
作者：
CHEN, QF;ARMSTRONG, K
通讯作者：
ARMSTRONG, K