Quercetin inhibits rhinovirus replication in vitro and in vivo.
Quercetin inhibits rhinovirus replication in vitro and in vivo.
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DOI：
10.1016/j.antiviral.2012.03.005
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发表时间：
2012-06
影响因子：
7.6
通讯作者：
Sajjan, Uma S.
中科院分区：
文献类型：
--
作者：
Ganesan, Shyamala;Faris, Andrea N.;Comstock, Adam T.;Wang, Qiong;Nanua, Suparna;Hershenson, Marc B.;Sajjan, Uma S.
鼻病毒是大多数普通感冒的原因。没有FDA批准的药物可用于治疗鼻病毒感染。我们发现，槲皮素，一种植物类黄酮阻断病毒在体外复制。此外，槲皮素抑制病毒刺激的细胞因子表达。槲皮素还能抑制病毒复制，减少体内肺部炎症。鼻病毒（RV）是大多数普通感冒的原因，也会导致哮喘和慢性阻塞性肺病患者病情加重。到目前为止，还没有药物可用于治疗鼻病毒感染。我们研究了槲皮素，一种植物黄烷醇对RV感染的影响，在体外和体内。用槲皮素预处理气道上皮细胞可降低Akt磷酸化、病毒内吞和IL-8反应。RV感染后6 h（病毒内吞作用后）添加槲皮素可降低气道上皮细胞中的病毒载量、IL-8和IFN反应。这与负链和正链病毒RNA和RV衣壳蛋白水平降低、RV诱导的eIF 4 GI切割消除和eIF 2 α磷酸化增加相关。在感染RV的小鼠中，槲皮素治疗降低了病毒复制以及趋化因子和细胞因子的表达。槲皮素治疗也减弱RV诱导的气道胆碱能高反应性。总之，我们的研究结果表明，槲皮素抑制RV内吞和复制在气道上皮细胞在多个阶段的RV生命周期。槲皮素还能降低RV感染小鼠的促炎细胞因子的表达，改善肺功能。基于这些观察结果，有必要进一步研究槲皮素在预防和治疗RV感染中的潜在益处。
► Rhinovirus is responsible for majority of common colds. ► There are no FDA approved drugs are available to treat rhinovirus infection. ► We show that quercetin, a plant flavonoid blocks viral replication in vitro. ► In addition quercetin inhibits virus-stimulated cytokine expression. ► Quercetin also inhibits viral replication and decreasing lung inflammation in vivo. Rhinovirus (RV), which is responsible for the majority of common colds, also causes exacerbations in patients with asthma and chronic obstructive pulmonary disease. So far, there are no drugs available for treatment of rhinovirus infection. We examined the effect of quercetin, a plant flavanol on RV infection in vitro and in vivo. Pretreatment of airway epithelial cells with quercetin decreased Akt phosphosphorylation, viral endocytosis and IL-8 responses. Addition of quercetin 6 h after RV infection (after viral endocytosis) reduced viral load, IL-8 and IFN responses in airway epithelial cells. This was associated with decreased levels of negative and positive strand viral RNA, and RV capsid protein, abrogation of RV-induced eIF4GI cleavage and increased phosphorylation of eIF2α. In mice infected with RV, quercetin treatment decreased viral replication as well as expression of chemokines and cytokines. Quercetin treatment also attenuated RV-induced airway cholinergic hyperresponsiveness. Together, our results suggest that quercetin inhibits RV endocytosis and replication in airway epithelial cells at multiple stages of the RV life cycle. Quercetin also decreases expression of pro-inflammatory cytokines and improves lung function in RV-infected mice. Based on these observations, further studies examining the potential benefits of quercetin in the prevention and treatment of RV infection are warranted.
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DOI：
10.1152/ajpregu.90319.2008
发表时间：
2008-08-01
影响因子：
2.8
作者：
Davis, J. M.;Murphy, E. A.;Gangemi, J. D.
通讯作者：
Gangemi, J. D.
影响因子：
7.1
作者：
HOLLMAN, PCH;DEVRIES, JHM;KATAN, MB
通讯作者：
KATAN, MB
影响因子：
4.8
作者：
Groskreutz, Dayna J.;Babor, Ellen C.;Hunninghake, Gary W.
通讯作者：
Hunninghake, Gary W.
影响因子：
7.3
作者：
DEMEYER, N;HAEMERS, A;VLIETINCK, AJ
通讯作者：
VLIETINCK, AJ
影响因子：
13.5
作者：
Gonzalez, Oscar;Fontanes, Vanessa;Raychaudhuri, Santanu;Loo, Rachel;Loo, Joseph;Arumugaswami, Vaithilingaraja;Sun, Ren;Dasgupta, Asim;French, Samuel W.
通讯作者：
French, Samuel W.