Ingested histamine and serotonin interact to alter Anopheles stephensi feeding and flight behavior and infection with Plasmodium parasites.
Ingested histamine and serotonin interact to alter Anopheles stephensi feeding and flight behavior and infection with Plasmodium parasites.
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DOI：
10.3389/fphys.2023.1247316
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发表时间：
2023
影响因子：
4
通讯作者：
Luckhart, Shirley
中科院分区：
文献类型：
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作者：
Coles, Taylor A. A.;Briggs, Anna M. M.;Hambly, Malayna G. G.;Cespedes, Nora;Fellows, Abigail M. M.;Kaylor, Hannah L. L.;Adams, Alexandria D. D.;Van Susteren, Grace;Bentil, Ronald E. E.;Robert, Michael A. A.;Riffell, Jeffrey A. A.;Lewis, Edwin E. E.;Luckhart, Shirley
关键词：
在人类疟疾中，组胺和5-羟色胺（5-HT）的血液水平发生了变化，在这些水平上，我们已经证明它们对斯氏按蚊在被这种入侵蚊子摄入后具有广泛的独立影响。考虑到组胺和5-HT在自然条件下被一起摄入，并且组胺能和组胺能信号在其他生物体中是网络化的，我们研究了这些生物胺的组合对A. stephensi在健康人水平（高5-HT，低组胺）或与严重疟疾相关的水平（低5-HT，高组胺）。在喂食A之前，在水中进行处理（引发）。stephensi对约氏疟原虫感染的小鼠或通过人工血粉。相对于组胺和5-HT单独的作用，生物胺组合的作用复杂。生物胺处理对第一个产卵周期的影响最大，高组胺缓和低5-HT的组合效果。相比之下，联合和单独给药的窝卵数相似。而高组胺单独增加未感染的A。Stephensi每周终生吸血，相对于对照，两种组合都没有改变这种趋势。第一次血餐后2周重新进食的趋势通过联合治疗改变，但这取决于分娩方式。对于血液输送，与健康相关的治疗相比，疟疾相关的治疗产生了更高的进食女性百分比，但对于预充则匡威。相对于对照和健康组合处理，用疟疾相关组合处理的雌性蚊子表现出增强的飞行行为和物体检查。与疟疾相关的组合引发的蚊子表现出较高的平均卵囊和子孢子感染率相对于健康的组合，与高组胺对这些模式具有主导作用。与未感染A. stephensi博士的研究表明，受感染蚊子吃第二顿血的倾向揭示了生物胺与感染的相互作用。我们使用了一个数学模型来预测不同水平的生物胺和相关变化对人群爆发的影响。虽然并非所有的爆发参数都受到相同的影响，但总的影响表明，组胺和5-HT改变了蚊子传播的可能性，这些蚊子以有症状的疟疾宿主和健康宿主为食。
Blood levels of histamine and serotonin (5-HT) are altered in human malaria, and, at these levels, we have shown they have broad, independent effects on Anopheles stephensi following ingestion by this invasive mosquito. Given that histamine and 5-HT are ingested together under natural conditions and that histaminergic and serotonergic signaling are networked in other organisms, we examined effects of combinations of these biogenic amines provisioned to A. stephensi at healthy human levels (high 5-HT, low histamine) or levels associated with severe malaria (low 5-HT, high histamine). Treatments were delivered in water (priming) before feeding A. stephensi on Plasmodium yoelii-infected mice or via artificial blood meal. Relative to effects of histamine and 5-HT alone, effects of biogenic amine combinations were complex. Biogenic amine treatments had the greatest impact on the first oviposition cycle, with high histamine moderating low 5-HT effects in combination. In contrast, clutch sizes were similar across combination and individual treatments. While high histamine alone increased uninfected A. stephensi weekly lifetime blood feeding, neither combination altered this tendency relative to controls. The tendency to re-feed 2 weeks after the first blood meal was altered by combination treatments, but this depended on mode of delivery. For blood delivery, malaria-associated treatments yielded higher percentages of fed females relative to healthy-associated treatments, but the converse was true for priming. Female mosquitoes treated with the malaria-associated combination exhibited enhanced flight behavior and object inspection relative to controls and healthy combination treatment. Mosquitoes primed with the malaria-associated combination exhibited higher mean oocysts and sporozoite infection prevalence relative to the healthy combination, with high histamine having a dominant effect on these patterns. Compared with uninfected A. stephensi, the tendency of infected mosquitoes to take a second blood meal revealed an interaction of biogenic amines with infection. We used a mathematical model to project the impacts of different levels of biogenic amines and associated changes on outbreaks in human populations. While not all outbreak parameters were impacted the same, the sum of effects suggests that histamine and 5-HT alter the likelihood of transmission by mosquitoes that feed on hosts with symptomatic malaria versus a healthy host.
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影响因子：
--
作者：
Donnelly E;de Water JV;Luckhart S
通讯作者：
Luckhart S
影响因子：
4
作者：
通讯作者：
--
影响因子：
2.2
作者：
LANGE, AB;ORCHARD, I;LLOYD, RJ
通讯作者：
LLOYD, RJ
影响因子：
4.7
作者：
LOO, YH
通讯作者：
LOO, YH
影响因子：
9.3
作者：
通讯作者：
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