Competence pheromone, oligopeptide permease, and induction of competence in Streptococcus pneumoniae
Competence pheromone, oligopeptide permease, and induction of competence in Streptococcus pneumoniae
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肺炎链球菌的能力信息素、寡肽通透酶和能力诱导
DOI：
10.1111/j.1365-2958.1996.tb02556.x
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发表时间：
1996
影响因子：
3.6
通讯作者：
J. Claverys
中科院分区：
文献类型：
--
作者：
Geneviève Alioing;Chantal Granadel;D. Morrison;J. Claverys
一种未经修饰的十七肽信息素能够引发肺炎链球菌的遗传转化能力，最近已被确定和表征。在考虑肽的可能信号转导机制时，先前表征的Ami寡肽通透酶和三种高度同源的寡肽结合脂蛋白AmiA。阿利亚和AliB似乎是受体的良好候选者。因此，我们比较了Ami、阿利亚和AliB突变体与同基因野生型菌株的自发转化能力，并研究了各种突变体对合成感受态刺激肽（CSP）处理的反应。我们的结果清楚地表明，Ami和迄今为止在S. pneumoniae的细胞是用合成CSP处理后感受态诱导所必需的。虽然不能完全排除存在第四个未鉴定的寡肽结合脂蛋白和/或第二个寡肽通透酶操纵子，但我们支持CSP信号传递涉及双组分调控系统的假设。尽管检测的单或双Ami和Ali突变体的感受态均未受到严重影响，但例外的alIB质粒插入突变完全消除了感受态。此外，三重AmiA-阿利亚-AliB突变体与野生型的不同之处在于没有显示出能力的尖峰，但在整个生长的指数期表现出可转化性。这些和以前的观察进行了讨论，并提出了一个一般性的假设，占调制的能力肽通透酶突变体在S。肺炎。
An unmodified heptadecapeptide pheromone capable of eliciting competence for genetic transformation in Streptococcus pneumoniae has recently been identified and characterized. In considering possible signaltransduction mechanisms for the peptide, the previously characterized Ami oligopeptide permease and the three highly homologous oligopeptide‐binding lipoproteins, AmiA. AliA, and AliB, appeared to be good candidates for receptors. We therefore compared the spontaneous transformability of Ami, AliA and AliB mutants to that of an isogenic wild‐type strain and we investigated the response of the various mutants to treatment with synthetic competence‐stimulating peptide (CSP). Our results clearly demonstrate that neither Ami nor any of the three highly homologous oligopeptide‐binding lipoproteins identified so far in S. pneumoniae are required for competence induction following treatment with synthetic CSP. Although the existence of a fourth unidentified oligopeptide‐binding lipoprotein and/or a second oligopeptide permease operon could not be completely ruled out, we favour the hypothesis that CSP signal transmission rather involves a two‐component regulatory system. Although none of the single or double Ami and Ali mutants tested appeared severely affected for competence, an exceptional aliB plasmid‐insertion mutation abolished competence completely. In addition, the triple AmiA‐AliA‐AliB mutant differed from wild type in showing no sharp peak of competence but exhibiting transformability throughout the exponential phase of growth. These and previous observations are discussed and a general hypothesis is proposed to account for the modulation of competence by peptide permease mutants in S. pneumoniae.
DOI：
10.1073/pnas.93.1.260
发表时间：
1996-01-09
影响因子：
11.1
作者：
Leonard, BAB;Podbielski, A;Dunny, GM
通讯作者：
Dunny, GM
收敛传感途径介导对枯草芽孢杆菌中两种细胞外能力因子的反应。
影响因子：
10.5
作者：
Solomon,JM;Magnuson,R;Srivastava,A;Grossman,AD
通讯作者：
Grossman,AD
影响因子：
10.5
作者：
Y. Weinrauch;R. Penchev;E. Dubnau;I. Smith;D. Dubnau
通讯作者：
Y. Weinrauch;R. Penchev;E. Dubnau;I. Smith;D. Dubnau