Genomic identification, rapid evolution, and expression of Argonaute genes in the tilapia, Oreochromis niloticus
Genomic identification, rapid evolution, and expression of Argonaute genes in the tilapia, Oreochromis niloticus
复制标题
罗非鱼 Argonaute 基因的基因组鉴定、快速进化和表达
DOI：
10.1007/s00427-016-0554-3
复制
发表时间：
2016-08
影响因子：
2.4
通讯作者：
Wang DS
中科院分区：
文献类型：
--
作者：
Sun LN;Chen JL;Shi HJ;Wang DS
Argonaute 蛋白是小 RNA 诱导的沉默复合物的关键成分，在 RNA 指导的调控途径中发挥多种作用。 Argonaute 基因可分为两个亚家族：Ago（与 microRNA/小干扰 RNA 相互作用）和 Piwi 亚家族（与 piwi 相互作用 RNA (piRNA) 相互作用）。在本研究中，全基因组分析首先鉴定了罗非鱼、腔棘鱼、斑点雀鳝和象鲨中的 Agos 和 Piwis 的不同成员。额外的硬骨鱼 Ago3b 是在鱼类特异性基因组复制事件之后产生的。对慈鲷和其他硬骨鱼之间 Agos 和 Piwis 的选择性压力分析表明，慈鲷谱系中 Piwil1 的进化加速，并且正选择位点位于 PIWI 结构域区域，表明这些氨基酸取代适应慈鲷信使 RNA (mRNA) 的靶向切割。与其他阶段相比，孵化后 5 天 (dah) 的 Ago1 和 Ago4 在卵巢和睾丸中检测到较高水平，支持之前报道的在早期胚胎发生期间需要 Ago 介导的途径来清除母体 mRNA 的要求。 Piwis 在罗非鱼睾丸中大量表达，表明它们在雄性种系中，特别是在精子发生中发挥重要作用。在骨骼肌中也检测到了 Piwis 的显着表达，表明 piRNA 途径可能不仅限于种系的发育和维持，而且可能在体细胞组织中发挥重要作用。通过定量 PCR (qPCR) 和原位杂交 (ISH) 检查 Piwil1 和 Piwil2 的表达，以验证空间和时间表达谱。总而言之，这些结果全面概述了罗非鱼 Argonaute 家族，并为该家族在硬骨鱼中的进化和功能提供了新的视角。
Argonaute proteins are key components of the small RNA-induced silencing complex and have multiple roles in RNA-directed regulatory pathways. Argonaute genes can be divided into two subfamilies: the Ago (interacting with microRNA/small interfering RNA) and Piwi subfamilies (interacting with piwi-interacting RNAs (piRNAs)). In the present study, genome-wide analyses firstly yielded the identification of different members of Agos and Piwis in the tilapia, coelacanth, spotted gar, and elephant shark. The additional teleost Ago3b was generated following the fish-specific genome duplication event. Selective pressure analysis on Agos and Piwis between cichlids and other teleosts showed an accelerated evolution of Piwil1 in the cichlid lineages, and the positive selected sites were located in the region of PIWI domain, suggesting that these amino acid substitutions are adapt to targeted cleavage of messenger RNA (mRNA) in cichlids. Ago1 and Ago4 were detected at higher levels at 5 days after hatching (dah) in both ovaries and testes compared with other stages, supporting the previously reported requirement of Ago-mediated pathways to clear the maternal mRNAs during the early embryogenesis. The Piwis were abundantly expressed in tilapia testes, indicating their essential roles in male germline, especially in spermatogenesis. Notable expression of Piwis was also detected in skeletal muscle, indicating that piRNA pathway may not only be confined to development and maintenance of the germline but may also play important roles in somatic tissues. The expression of Piwil1 and Piwil2 was examined by quantitative PCR (qPCR) and in situ hybridization (ISH) to validate the spatial and temporal expression profiles. Taken together, these results present a thorough overview of tilapia Argonaute family and provide a new perspective on the evolution and function of this family in teleosts.
登录
查看更多内容
性逆转过程中小RNA的动态进化和生物发生
影响因子：
4.6
作者：
Liu J;Luo M;Sheng Y;Hong Q;Cheng H;Zhou R
通讯作者：
Zhou R
影响因子：
2.9
作者：
P. C. Shekar;Adnan Naim;D. P. Sarathi;Satish Kumar
通讯作者：
P. C. Shekar;Adnan Naim;D. P. Sarathi;Satish Kumar
影响因子：
3.5
作者：
Fablet, Marie;Akkouche, Abdou;Vieira, Cristina
通讯作者：
Vieira, Cristina
影响因子：
10.7
作者：
Kolaczkowski, Bryan;Hupalo, Daniel N.;Kern, Andrew D.
通讯作者：
Kern, Andrew D.
影响因子：
3.5
作者：
Qiu-Yang Yao;En-Hua Xia;Feihu Liu;Li-zhi Gao
通讯作者：
Qiu-Yang Yao;En-Hua Xia;Feihu Liu;Li-zhi Gao