Death of tonsillar B cells by NETosis.
Death of tonsillar B cells by NETosis.
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DOI：
10.1038/s41420-023-01402-4
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发表时间：
2023-03-30
影响因子：
7
通讯作者：
Knight KL
中科院分区：
文献类型：
--
作者：
Bukhari A;Kalinina O;Knight KL
调节B细胞死亡对于产生抗体和维持免疫耐受是必不可少的。B细胞可因凋亡而死亡，我们报道人扁桃体B细胞，但外周血B细胞不会因NETosis而死亡。这种细胞死亡是密度依赖性的，其特征在于细胞和核膜完整性的丧失、活性氧的释放和染色质去浓缩。扁桃体B细胞分泌高水平的TNF，抑制TNF可防止染色质去凝聚。通过原位荧光显微镜，B细胞NETosis，如通过组蛋白-3的高瓜氨酸化鉴定的，定位于正常扁桃体中生发中心的亮区（LZ），并与B细胞标志物CD 19/IgM重叠。我们提出了一个模型，其中刺激LZ中的B细胞诱导NETosis，部分由TNF驱动。我们还提供了证据表明扁桃体B细胞的NETosis可能被扁桃体中一种未知的因子抑制。这些结果描述了一种以前未鉴定的B细胞死亡形式，并提出了一种在免疫应答期间维持B细胞稳态的新机制。
Regulating B cell death is essential for generating antibodies and maintaining immune tolerance. B cells can die by apoptosis, and we report that human tonsil B cells, but not peripheral blood B cells also die by NETosis. This cell death is density-dependent, characterized by the loss of cell and nuclear membrane integrity, release of reactive oxygen species, and chromatin decondensation. Tonsil B cells secrete high levels of TNF, and inhibiting TNF prevented chromatin decondensation. By in situ fluorescence microscopy, B cell NETosis, as identified by the hyper citrullination of Histone-3, was localized to the light zone (LZ) of germinal centers in normal tonsil and overlapped with the B cell marker CD19/IgM. We propose a model in which stimulation of B cells in the LZ induces NETosis, driven in part by TNF. We also provide evidence that NETosis of tonsil B cells may be inhibited by an unidentified factor in tonsil. The results describe a previously unidentified form of B cell death and suggest a new mechanism to maintain B cell homeostasis during immune responses.
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DOI：
10.1126/science.aao4227
发表时间：
2018-09-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Albrengues J;Shields MA;Ng D;Park CG;Ambrico A;Poindexter ME;Upadhyay P;Uyeminami DL;Pommier A;Küttner V;Bružas E;Maiorino L;Bautista C;Carmona EM;Gimotty PA;Fearon DT;Chang K;Lyons SK;Pinkerton KE;Trotman LC;Goldberg MS;Yeh JT;Egeblad M
通讯作者：
Egeblad M
影响因子：
1.3
作者：
Marionneaux, Steven M.;Keohane, Elaine M.;Mehta, Shashi R.
通讯作者：
Mehta, Shashi R.
影响因子：
14.8
作者：
Lewis, Huw D.;Liddle, John;Coote, Jim E.;Atkinson, Stephen J.;Barker, Michael D.;Bax, Benjamin D.;Bicker, Kevin L.;Bingham, Ryan P.;Campbell, Matthew;Chen, Yu Hua;Chung, Chun-Wa;Craggs, Peter D.;Davis, Rob P.;Eberhard, Dirk;Joberty, Gerard;Lind, Kenneth E.;Locke, Kelly;Maller, Claire;Martinod, Kimberly;Patten, Chris;Polyakova, Oxana;Rise, Cecil E.;Ruediger, Martin;Sheppard, Robert J.;Slade, Daniel J.;Thomas, Pamela;Thorpe, Jim;Yao, Gang;Drewes, Gerard;Wagner, Denisa D.;Thompson, Paul R.;Prinjha, Rab K.;Wilson, David M.
通讯作者：
Wilson, David M.
影响因子：
2.7
作者：
Johansson, P.;Eisele, L.;Nueckel, H.
通讯作者：
Nueckel, H.
影响因子：
3.4
作者：
Lunning, MA;Zenger, VE;Marti, GE
通讯作者：
Marti, GE