Inhibition of Peripheral ERK Signaling Ameliorates Persistent Muscle Pain Around Trigger Points in Rats.
Inhibition of Peripheral ERK Signaling Ameliorates Persistent Muscle Pain Around Trigger Points in Rats.
复制标题
抑制外周 ERK 信号可改善大鼠触发点周围的持续性肌肉疼痛。
DOI：
10.1177/0963689720960190
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发表时间：
2020-01
影响因子：
3.3
通讯作者：
Qi F
中科院分区：
文献类型：
--
作者：
Zhu YC;Jin FH;Zhang MY;Qi F
本研究的目的是探讨ERK信号通路是否参与改善大鼠腓肠肌挫伤后慢性肌筋膜痛敏。我们建立了一种与肌筋膜疼痛综合征相关的动物模型，并描述了动物模型中肌肉疼痛的机制。在大鼠腓肠肌肌筋膜触发点周围注射ERK抑制剂后0.5、1、2、3、4、5、8、12、18和24 h观察机械痛阈的变化。苏木精-伊红（H&E）染色观察腓肠肌细胞形态学变化。通过免疫组化和Western blotting检测ERK信号通路的激活。损伤肌纤维在横截面上呈大小不等的圆形或椭圆形聚集，在纵截面上呈连续的膨胀状和锥形。肌肉注射ERK抑制剂U 0126后，机械痛阈显著升高。机械性痛觉过敏的减少伴随着ERK蛋白磷酸化、肌球蛋白轻链激酶（MLCK）蛋白、p-MLC蛋白表达和MTrPs周围骨骼肌细胞横截面积的减少。ERK抑制剂有助于减轻大鼠肌筋膜疼痛模型中的机械性痛觉过敏，并且痛阈的增加可能与ERK下调MLCK和其他相关收缩相关蛋白有关。
The purpose of this study was to investigate whether the ERK signaling pathway was involved in ameliorating chronic myofascial hyperalgesia from contused gastrocnemius muscle in rats. We established an animal model associated with myofascial pain syndrome and described the mechanism of muscle pain in an animal model. Changes in the mechanical pain threshold were observed 0.5, 1, 2, 3, 4, 5, 8, 12, 18, and 24 h after ERK inhibitor injection around myofascial trigger points (MTrPs) of the gastrocnemius muscle in rats. Morphological changes in gastrocnemius muscle cells were observed by hematoxylin and eosin (H&E) staining. ERK signaling pathway activation was detected through immunohistochemistry and Western blotting. The main morphological characteristics of injured muscle fibers around MTrPs include gathered circular or elliptical shapes of different sizes in the cross-section and continuous inflated and tapering fibers in the longitudinal section. After intramuscular injection of U0126 (ERK inhibitor), the mechanical pain threshold significantly increased. The reduction in mechanical hyperalgesia was accompanied by reduced ERK protein phosphorylation, myosin light chain kinase (MLCK) protein, p-MLC protein expression, and the cross-sectional area of skeletal muscle cells around MTrPs. An ERK inhibitor contributed to the attenuation of mechanical hyperalgesia in the rat myofascial pain model, and the increase in pain threshold may be related to MLCK downregulation and other related contraction-associated proteins by ERK.
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DOI：
10.1196/annals.1363.013
发表时间：
2005-01-01
期刊：
CELL INJURY: MECHANISMS, RESPONSES, AND REPAIR
影响因子：
--
作者：
Gissel, Hanne
通讯作者：
Gissel, Hanne
影响因子：
4.8
作者：
Ryder, Jeffrey W.;Lau, Kim S.;Stull, James T.
通讯作者：
Stull, James T.
影响因子：
4.8
作者：
Adi, S;Bin-Abbas, B;Rosenthal, SM
通讯作者：
Rosenthal, SM
影响因子：
3.3
作者：
Margalef, Ramon;Sisquella, Marc;Santafe, Manel M.
通讯作者：
Santafe, Manel M.
影响因子：
7.4
作者：
Alvarez P;Bogen O;Green PG;Levine JD
通讯作者：
Levine JD