Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia.
Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia.
复制标题
DOI：
10.1371/journal.pone.0246760
复制
发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Franz-Montan M
中科院分区：
文献类型：
--
作者：
Batista da Silva C;Volpato MC;Muniz BV;Dos Santos CP;Serpe L;Ferreira LEN;de Melo NFS;Fraceto LF;Groppo FC;Franz-Montan M
为确定将阿替卡因（ATC）包裹在聚（ε - 己内酯）纳米胶囊（ATCnano）中是否可提高其渗透能力和镇痛效果，并降低细胞毒性，旨在用于牙科的局部或表面麻醉。在将HaCaT细胞暴露于ATC、ATCnano、含肾上腺素的ATC（ATCepi）以及含肾上腺素的纳米胶囊中的ATC（ATCnanoepi）1小时和24小时后，评估细胞活力（使用MTT试验和荧光显微镜）。使用Franz型垂直扩散池测定2% ATC和2% ATCnano穿过猪食管上皮的渗透曲线。在大鼠术后疼痛模型中，使用von Frey麻醉仪评估镇痛效果，将2% ATC、2% ATCnano、2% ATCepi和2% ATCnanoepi配方与4% ATCepi（一种市售配方）进行比较。我们表明，使用纳米胶囊降低了阿替卡因的毒性（P < 0.0001）并增加了其通量（P = 0.0007）。与2% ATC、2% ATCnano和2% ATCnanoepi相比，2% ATCepi和4% ATCepi配方提供了更高的镇痛成功率和持续时间（P < 0.05）。载有阿替卡因的聚（ε - 己内酯）纳米胶囊是一种有前途的口腔内表面麻醉配方（在局部麻醉注射之前），尽管在炎症组织（如不可逆牙髓炎）中注射用于疼痛控制时无效。
To determine whether the permeation capacity and analgesic efficacy of articaine (ATC) could be increased and cytotoxicity decreased by encapsulation in poly(ɛ-caprolactone) nanocapsules (ATCnano), aiming at local or topical anesthesia in dentistry. Cellular viability was evaluated (using the MTT test and fluorescence microscopy) after 1 h and 24 h exposure of HaCaT cells to ATC, ATCnano, ATC with epinephrine (ATCepi), and ATC in nanocapsules with epinephrine (ATCnanoepi). The profiles of permeation of 2% ATC and 2% ATCnano across swine esophageal epithelium were determined using Franz-type vertical diffusion cells. Analgesic efficacy was evaluated with a von Frey anesthesiometer in a postoperative pain model in rats, comparing the 2% ATC, 2% ATCnano, 2% ATCepi, and 2% ATCnanoepi formulations to 4% ATCepi (a commercially available formulation). We show that use of the nanocapsules decreased the toxicity of articaine (P<0.0001) and increased its flux (P = 0.0007). The 2% ATCepi and 4% ATCepi formulations provided higher analgesia success and duration (P<0.05), compared to 2% ATC, 2% ATCnano, and 2% ATCnanoepi. Articaine-loaded poly(ɛ-caprolactone) nanocapsules constitute a promising formulation for intraoral topical anesthesia (prior to local anesthetic injection), although it is not effective when injected in inflamed tissues for pain control, such as irreversible pulpitis.
登录
查看更多内容
影响因子：
2.7
作者：
Boonkaew, Benjawan;Kempf, Margit;Cuttle, Leila
通讯作者：
Cuttle, Leila
DOI：
10.14219/jada.archive.2001.0152
发表时间：
2001-02-01
影响因子：
3.9
作者：
Malamed, SF;Gagnon, S;Leblanc, D
通讯作者：
Leblanc, D
影响因子：
4.2
作者：
Ashraf, Hengameh;Kazem, Majeed;Noghrehkar, Fatemeh
通讯作者：
Noghrehkar, Fatemeh
DOI：
10.1016/j.ejpb.2011.04.009
发表时间：
2011-09-01
影响因子：
4.9
作者：
Abdel-Mottaleb, Mona M. A.;Neumann, Dirk;Lamprecht, Alf
通讯作者：
Lamprecht, Alf
影响因子：
3.3
作者：
Nunes Ferreira, Luiz Eduardo;Muniz, Bruno Vilela;Groppo, Francisco Carlos
通讯作者：
Groppo, Francisco Carlos