T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.
T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.
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T细胞子集由Lyt-1,2,3和THY-1抗原的表达定义。单克隆抗体的两参数免疫荧光和细胞毒性分析可修饰当前视图。
DOI：
10.1084/jem.152.2.280
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发表时间：
1980-08-01
期刊：
影响因子：
--
通讯作者：
Herzenberg LA
中科院分区：
文献类型：
--
作者：
Ledbetter JA;Rouse RV;Micklem HS;Herzenberg LA
利用单克隆抗体和多参数荧光分析，我们发现Lyt-1、Lyt-2和Lyt-3在T细胞亚群上的表达比用常规试剂定义Lyt-1+2+3+、Lyt-1+2-3-和Lyt-1-2+3+群体的细胞毒耗光研究最初认识到的更为复杂。我们在所有T淋巴细胞上至少检测到一些Lyt-1；然而，与之前的研究一致，我们发现Lyt-2+3+细胞比Lyt-1+2-3-细胞更难被抗Lyt-1清除。令人惊讶的是，我们发现一小部分细胞亚群携带相对大量的Lyt-1，而荧光激活细胞分选分析无法检测到Thy-1。我们也在B细胞区（原发卵泡）和脾脏和淋巴结的生发中心检测到这种表型的细胞。一般来说，仅Lyt-1的群体约占脾细胞的2%。Thy-1、Lyt- 1、Lyt-2和Lyt-3的相对定量表达在T细胞成熟过程中有系统的变化。在胸腺的Lyt-1+2+3+细胞中，Thy-1和Lyt-2高，Lyt-1低。脾T细胞中Thy-1较低，Lyt-1较高，Lyt-2和Lyt-3略高于胸腺。一般情况下，Thy-1表达与Lyt-1呈负相关。因此，即使在脾脏和淋巴结的T细胞亚群中，也存在高Thy-1和低Lyt-1的倾向，反之亦然。Lyt-2+3+细胞约占胸腺细胞的85%，但仅占脾或淋巴结T细胞的35%。Lyt-2+3+细胞主要存在于Lyt-1低、Thy-1高的亚群中。
Using monoclonal antibodies and multiparameter fluorescence analyses, we show that the expression of Lyt-1, Lyt-2, and Lyt-3 on T cell subpopulations is more complex than was originally recognized by the cytotoxic depletion studies with conventional reagents that defined the Lyt-1+2+3+, Lyt-1+2-3-, and Lyt-1-2+3+ populations. We detect at least some Lyt-1 on all T (Thy-1-bearing) lymphocytes; however, in agreement with previous studies, we find that Lyt-2+3+ cells are more difficult to depelete with anti-Lyt-1 than Lyt-1+2-3- cells. Surprisingly, we found a small subpopulation of cells carrying relatively large amounts of Lyt-1 and no Thy-1 detectable by fluorescence-activated cell sorter analysis. We also detect cells with this phenotype histologically in B cell zones (primary follicles) and germinal centers in spleen and lymph nodes. In general, the Lyt-1 only population represents approximately 2% of spleen cells. The relative quantitative expression of Thy-1, Lyt- 1, Lyt-2, and Lyt-3 changes systematically during T cell maturation. Among Lyt-1+2+3+ cells in the thymus, Thy-1 and Lyt-2 are high, whereas Lyt-1 is low. Among splenic T cells, in contrast, Thy-1 is low, Lyt-1 is high, and Lyt-2 and Lyt-3 are a little higher than in thymus. In general, Thy-1 expression is negatively correlated with Lyt-1. Thus, even among splenic and lymph node T cells subpopulations exist that tend to be either high Thy-1 and low Lyt-1 or vice versa. Lyt-2+3+ cells represent approximately 85% of thymocytes but only approximately 35% of splenic or lymph node T cells. The Lyt-2+3+ cells are found predominantly in the low Lyt-1, high Thy-1 subpopulation.
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DOI：
10.1084/jem.141.6.1376
发表时间：
1975-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cantor H;Boyse EA
通讯作者：
Boyse EA
同种异体反应性的生物学意义。 T细胞的个体发育集对同种抗原或改良的自抗原。
影响因子：
15.3
作者：
Burakoff, S J;Finberg, R;Glimcher, L;Lemonnier, F;Benacerraf, B;Cantor, H
通讯作者：
Cantor, H
影响因子：
5.4
作者：
JULIUS, MH;SIMPSON, E;HERZENBERG, LA
通讯作者：
HERZENBERG, LA
影响因子：
4.3
作者：
CANTOR, H;SIMPSON, E;HERZENBERG, LA
通讯作者：
HERZENBERG, LA
影响因子：
4.3
作者：
FATHMAN, CG;SMALL, M;WEISSMAN, IL
通讯作者：
WEISSMAN, IL