Titanium Dioxide Nanoparticle Ingestion Alters Nutrient Absorption in an In Vitro Model of the Small Intestine.
Titanium Dioxide Nanoparticle Ingestion Alters Nutrient Absorption in an In Vitro Model of the Small Intestine.
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DOI：
10.1016/j.impact.2017.01.002
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发表时间：
2017-01
期刊：
影响因子：
4.9
通讯作者：
Mahler GJ
中科院分区：
文献类型：
--
作者：
Guo Z;Martucci NJ;Moreno-Olivas F;Tako E;Mahler GJ
从农用化学品、加工食品和营养补充剂等产品中摄入二氧化钛 (TiO2) 纳米颗粒几乎是不可避免的。胃肠道是人体与外界环境之间的重要界面，也是吸收必需营养物质的场所。本研究的目的是通过小肠上皮体外细胞培养模型检查摄入 30 nm TiO2 纳米颗粒的效果，并确定急性或慢性暴露于纳米 TiO2 如何影响肠道屏障功能、活性氧生成、促炎信号、营养吸收（铁、锌、脂肪酸）和刷状缘膜酶功能（肠碱性磷酸酶）。将 Caco-2/HT29-MTX 细胞培养模型短期（四小时）或长期（五天）暴露于生理相关剂量的 TiO2 纳米粒子。长期接触二氧化钛纳米颗粒会显着降低肠道屏障功能。活性氧 (ROS) 的产生、促炎信号传导和肠道碱性磷酸酶活性均显示出对纳米 TiO2 的响应有所增加。暴露于 TiO2 纳米粒子后，铁、锌和脂肪酸的转运显着减少。这是因为纳米颗粒暴露导致肠上皮细胞中吸收性微绒毛的减少。营养转运蛋白基因表达也发生了改变，这表明细胞正在努力调节因纳米颗粒摄入而扰乱的转运机制。总的来说，这些结果表明，肠上皮细胞在功能水平上受到生理相关的暴露于通常从食物中摄入的纳米颗粒的影响。
Ingestion of titanium dioxide (TiO2) nanoparticles from products such as agricultural chemicals, processed food, and nutritional supplements is nearly unavoidable. The gastrointestinal tract serves as a critical interface between the body and the external environment, and is the site of essential nutrient absorption. The goal of this study was to examine the effects of ingesting the 30 nm TiO2 nanoparticles with an in vitro cell culture model of the small intestinal epithelium, and to determine how acute or chronic exposure to nano-TiO2 influences intestinal barrier function, reactive oxygen species generation, proinflammatory signaling, nutrient absorption (iron, zinc, fatty acids), and brush border membrane enzyme function (intestinal alkaline phosphatase). A Caco-2/HT29-MTX cell culture model was exposed to physiologically relevant doses of TiO2 nanoparticles for acute (four hours) or chronic (five days) time periods. Exposure to TiO2 nanoparticles significantly decreased intestinal barrier function following chronic exposure. Reactive oxygen species (ROS) generation, proinflammatory signaling, and intestinal alkaline phosphatase activity all showed increases in response to nano-TiO2. Iron, zinc, and fatty acid transport were significantly decreased following exposure to TiO2 nanoparticles. This is because nanoparticle exposure induced a decrease in absorptive microvilli in the intestinal epithelial cells. Nutrient transporter protein gene expression was also altered, suggesting that cells are working to regulate the transport mechanisms disturbed by nanoparticle ingestion. Overall, these results show that intestinal epithelial cells are affected at a functional level by physiologically relevant exposure to nanoparticles commonly ingested from food.
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影响因子：
5
作者：
De Angelis, Isabella;Barone, Flavia;De Berardis, Barbara
通讯作者：
De Berardis, Barbara
DOI：
10.1152/ajpgi.2001.280.5.g922
发表时间：
2001-05-01
影响因子：
4.5
作者：
Atuma, C;Strugala, V;Holm, L
通讯作者：
Holm, L
DOI：
10.1016/0006-291x(91)91647-u
发表时间：
1991-03-29
影响因子：
3.1
作者：
ARTURSSON, P;KARLSSON, J
通讯作者：
KARLSSON, J
影响因子：
15.9
作者：
Choe, Kibaek;Jang, Jeon Yeob;Kim, Pilhan
通讯作者：
Kim, Pilhan
影响因子：
8.8
作者：
Galy, Bruno;Ferring-Appel, Dunja;Hentze, Matthias W.
通讯作者：
Hentze, Matthias W.