TGF‐β1 stimulates cultured human fibroblasts to proliferate and produce tissue‐like fibroplasia: A fibronectin matrix‐dependent event
TGF‐β1 stimulates cultured human fibroblasts to proliferate and produce tissue‐like fibroplasia: A fibronectin matrix‐dependent event
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TGF-β1 刺激培养的人成纤维细胞增殖并产生组织样纤维增生：纤连蛋白基质依赖性事件
DOI：
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发表时间：
1997
影响因子：
5.6
通讯作者：
J. McPherson
中科院分区：
文献类型：
--
作者：
R. Clark;G. McCoy;J. Folkvord;J. McPherson
During wound repair, fibroblasts accumulate in the injured area until any defect is filled with stratified layers of cells and matrix. Such fibroplasia also occurs in many fibrotic disorders. Transforming growth factor‐β (TGF‐β), a promotor of granulation tissue in vivo and extracellular matrix production in vitro, is expressed during the active fibroplasia of wound healing and fibroproliferative diseases. Under usual tissue culture conditions, normal fibroblasts grow to confluence and then cease proliferation. In this study, culture conditions with TGF‐β1 have been delineated that promote human fibroblasts to grow in stratified layers mimicking in vivo fibroplasia. When medium supplemented with serum, ascorbate, proline, and TGF‐β was added thrice weekly to normal human dermal fibroblasts, the cells proliferated and stratified up to 16 cell layers thick within the culture dish, producing a tissue‐like fibroplasia. TGF‐β stimulated both DNA synthesis as measured by 1H‐thymidine uptake and cell proliferation as measured by a Hoechst dye DNA assay in these postconfluent cultures. The stratification was dependent on fibronectin assembly, as demonstrated by anti‐fibronectin antibodies which inhibited both basal and TGF‐β‐stimulated cell proliferation and stratification. Suppression of collagen matrix assembly in cell layers with β‐amino‐proprionitrile (BAPN) did not inhibit basal or TGF‐β stimulated in vitro fibroplasia. BAPN did not interfere with fibronectin matrix assembly as judged by immunofluorescence microscopy. Thus, in concert with serum factors, TGF‐β stimulates postconfluent, fibronectin matrix‐dependent, fibroblast growth creating a fibroplasia‐like tissue in vitro. J Cell Physiol 170:69–80, 1997 © 1997 Wiley‐Liss, Inc.
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影响因子：
56.9
作者：
HEIMARK, RL;TWARDZIK, DR;SCHWARTZ, SM
通讯作者：
SCHWARTZ, SM
DOI：
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发表时间：
1986-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;J. Massagué
通讯作者：
R. Ignotz;J. Massagué
DOI：
--
发表时间：
1991
期刊：
The American journal of pathology
影响因子：
--
作者：
Rae Merwin;W. Newman;L. Dawson;BeaIl;A. Tucker;J. Madri
通讯作者：
Rae Merwin;W. Newman;L. Dawson;BeaIl;A. Tucker;J. Madri
血管损伤患者血浆 ED1（“细胞”）纤连蛋白水平升高。
DOI：
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发表时间：
1989
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Peters,JH;Maunder,RJ;Woolf,AD;Cochrane,CG;Ginsberg,MH
通讯作者：
Ginsberg,MH
人内皮细胞合成、加工和分泌具有可变剪接 III 型同源性 (ED1) 的纤连蛋白分子。
影响因子：
20.3
作者：
Peters,JH;Sporn,LA;Ginsberg,MH;Wagner,DD
通讯作者：
Wagner,DD