Lipid-bilayer-spanning DNA nanopores with a bifunctional porphyrin anchor.
Lipid-bilayer-spanning DNA nanopores with a bifunctional porphyrin anchor.
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DOI：
10.1002/anie.201305765
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发表时间：
2013-11-11
影响因子：
16.6
通讯作者：
Howorka, Stefan
中科院分区：
文献类型：
--
作者：
Burns, Jonathan R.;Goepfrich, Kerstin;Wood, James W.;Thacker, Vivek V.;Stulz, Eugen;Keyser, Ulrich F.;Howorka, Stefan
化学在增强DNA纳米技术的能力方面处于强大的地位。[1，2] DNA折纸提供了合理设计的纳米级结构框架，[2，3]然而化学可以通过使用化学标签选择性修饰核酸来添加定制的功能来推进它们。这种协同方法有助于创造新的纳米级器件，从能够结合蛋白质的亲和纳米阵列[4]和量子点[5]到用于单分子光化学的纳米平台[6]以及用于生物传感的分子显示剂。[7，8]在本文中，我们提出了一种独特的化学策略，用于扩大和富集由折叠DNA组成的新兴跨膜纳米孔。[9，10]我们表明，只有两个基于卟啉的疏水标签实现了将高度带负电荷的DNA纳米结构锚定到脂质双层的疏水核心中的能量不利锚定。这种非常少量的卟啉标签大大简化了目前可用于纳米孔双层锚定的化学策略。芳香族卟啉标签也具有荧光性，因此有助于基于DNA的膜通道的显微镜可视化。我们的双功能化学标签的通用路线可能适用于许多其他DNA设计，并将有助于扩大实验获得多功能DNA折纸孔。
Chemistry is in a powerful position to enhance the capabilities of DNA nanotechnology.[1, 2] DNA origami offers rationally designed nanoscale structural frameworks,[2, 3] yet chemistry can advance them by adding tailored functionality through the selective modification of nucleic acids with chemical tags. This synergistic approach has helped create new nanoscale devices, ranging from affinity nanoarrays capable of binding proteins [4] and quantum dots,[5] to nanoplatforms for singlemolecule photochemistry,[6] and to molecular display agents for biosensing.[7, 8] Herein, we present a unique chemical strategy for enlarging and enriching the emerging class of membrane-spanning nanopores composed of folded DNA.[9, 10] We show that solely two porphyrin-based hydrophobic tags achieve the otherwise energetically unfavorable anchoring of the highly negatively charged DNA nanostructure into the hydrophobic core of lipid bilayers. This very small number of porphyrin tags considerably simplifies the currently available chemical strategies for bilayer anchoring of nanopores. The aromatic porphyrin tags are also fluorescent, and hence facilitate the microscopic visualization of DNA-based membrane channels. Our generic route for dualfunctional chemical tags can likely be applied to many other DNA designs, and will help broaden experimental access to versatile DNA origami pores.
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影响因子：
14.9
作者：
Douglas SM;Marblestone AH;Teerapittayanon S;Vazquez A;Church GM;Shih WM
通讯作者：
Shih WM
影响因子：
10.8
作者：
Ke, Yonggang;Sharma, Jaswinder;Yan, Hao
通讯作者：
Yan, Hao
影响因子：
3.2
作者：
Brewer, Ashley;Siligardi, Guiliano;Stulz, Eugen
通讯作者：
Stulz, Eugen
影响因子：
3.9
作者：
Goepfrich, Kerstin;Kulkarni, Chandrashekhar V.;Keyser, Ulrich F.
通讯作者：
Keyser, Ulrich F.
影响因子：
17.1
作者：
Leitner, Michael;Mitchell, Nick;Ebner, Andreas
通讯作者：
Ebner, Andreas