Characterization of muscle-regulatory gene, MyoD, from flounder (Paralichthys olivaceus) and analysis of its expression patterns during embryogenesis.
Characterization of muscle-regulatory gene, MyoD, from flounder (Paralichthys olivaceus) and analysis of its expression patterns during embryogenesis.
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DOI：
10.1007/s10126-005-5042-0
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发表时间：
2006-03
影响因子：
3
通讯作者：
Xu, YL
中科院分区：
文献类型：
--
作者：
Zhang, YQ;Tan, XA;Zhang, PJ;Xu, YL
骨骼肌细胞的特化和分化是由肌源性调节因子(MRF)编码成员的基因活性驱动的。在脊椎动物中，MRF家族包括MyoD、Myf5、Mygenin和MRF4。MRF能够将各种非肌肉细胞转化为成肌细胞和肌管。为了更好地了解它们在鱼类肌肉发育中的作用，我们从牙鲆(Paralichthys Olivaceus)中分离了MyoD基因，并对其结构和表达模式进行了分析。序列分析表明，比目鱼MyoD的结构与脊椎动物的MRF相似，具有三个外显子和两个内含子，其蛋白含有一个高度保守的碱性螺旋-环-螺旋结构域(BHLH)。序列比较表明，比目鱼MyoD基因与其他鱼类MyoD基因高度保守。序列比对和系统发育分析表明，牙鲆MyoD、金枪鱼MyoD、红鳍罗非鱼MyoD和罗非鱼MyoD可能是同源基因。首次检测到比目鱼MyoD的表达为节段板中的两排分裂前细胞。在体细胞发生过程中，MyoD转录本存在于产生慢肌的近轴细胞和产生快肌的外侧体细胞中。30个体节形成后，除尾部体节外，其余体节MyoD表达均下降，与体节成熟一致。在孵化阶段，MyoD在其他肌细胞和尾状体节中表达。它只在生长中的鱼的肌肉中检测到。
Specification and differentiation of skeletal muscle cells are driven by the activity of genes encoding members of the myogenic regulatory factors (MRFs). In vertebrates, the MRF family includes MyoD, Myf5, myogenin, and MRF4. The MRFs are capable of converting a variety of nonmuscle cells into myoblasts and myotubes. To better understand their roles in fish muscle development, we isolated the MyoD gene from flounder (Paralichthys olivaceus) and analyzed its structure and patterns of expression. Sequence analysis showed that flounder MyoD shared a structure similar to that of vertebrate MRFs with three exons and two introns, and its protein contained a highly conserved basic helix–loop–helix domain (bHLH). Comparison of sequences revealed that flounder MyoD was highly conserved with other fish MyoD genes. Sequence alignment and phylogenetic analysis indicated that flounder MyoD, seabream (Sparus aurata) MyoD1, takifugu (Takifugu rubripes) MyoD, and tilapia (Oreochromis aureus) MyoD were more likely to be homologous genes. Flounder MyoD expression was first detected as two rows of presomitic cells in the segmental plate. From somitogenesis, MyoD transcripts were present in the adaxial cells that give rise to slow muscles and the lateral somitic cells that give rise to fast muscles. After 30 somites formed, MyoD expression decreased in the somites except the caudal somites, coincident with somite maturation. In the hatching stage, MyoD was expressed in other muscle cells and caudal somites. It was detected only in muscle in the growing fish.
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影响因子：
4.8
作者：
Langlands, K;Yin, XY;Prochownik, EV
通讯作者：
Prochownik, EV
影响因子：
14.9
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Kel-Margoulis, OV;Kel, AE;Wingender, E
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Wingender, E
影响因子：
2.4
作者：
Delalande, JM;Rescan, PY
通讯作者：
Rescan, PY
影响因子：
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作者：
Gerber, AN;Klesert, TR;Tapscott, SJ
通讯作者：
Tapscott, SJ
影响因子：
4
作者：
Arnold, HH;Winter, B
通讯作者：
Winter, B