First Report of Wenzhou sobemo-like virus 4 in Aedes albopictus (Diptera: Culicidae) in Latin America.
First Report of Wenzhou sobemo-like virus 4 in Aedes albopictus (Diptera: Culicidae) in Latin America.
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DOI：
10.3390/v14112341
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发表时间：
2022-10-25
期刊：
影响因子：
--
通讯作者：
Lima-Camara TN
中科院分区：
文献类型：
--
作者：
Andrade PS;Valença IN;Heinisch MRS;Rocha EC;Fernandes LN;Faria NR;Sabino EC;Lima-Camara TN
昆虫特异性病毒（ISV）是只在节肢动物细胞中复制的病毒。已经在蚊子中研究了许多ISV，因为它们中的许多充当人类病原体（例如虫媒病毒）的载体。白纹伊蚊（Stegomyia）albopictus是巴西登革热（DENV）、寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV）等几种虫媒病毒的重要潜在载体。下一代测序宏基因组学的发展使新的ISV的发现和表征成为可能。AE.使用放置在巴西圣保罗市的两个城市公园中的产卵陷阱收集白纹伊蚊卵。将白纹伊蚊雌性分成池，提取遗传物质并进行处理以通过宏基因组学进行测序。在检测的4个库中的3个中获得了ISV温州sobemo-like病毒4（WSLV 4）的完整基因组。这是首次在Ae中检测到ISV WSLV 4。拉丁美洲的雌性白纹伊蚊进一步研究了Ae.为了更好地了解该物种在美洲虫媒病毒传播动态中的作用，需要对白纹伊蚊进行研究。
Insect-specific viruses (ISVs) are viruses that replicate exclusively in arthropod cells. Many ISVs have been studied in mosquitoes as many of them act as vectors for human etiological agents, such as arboviruses. Aedes (Stegomyia) albopictus is an important potential vector of several arboviruses in Brazil, such as dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV). The development of next-generation sequencing metagenomics has enabled the discovery and characterization of new ISVs. Ae. albopictus eggs were collected using oviposition traps placed in two urban parks in the city of São Paulo, Brazil. The Aedes albopictus females were divided into pools and the genetic material was extracted and processed for sequencing by metagenomics. Complete genomes of ISV Wenzhou sobemo-like virus 4 (WSLV4) were obtained in three of the four pools tested. This is the first detection of ISV WSLV4 in Ae. albopictus females in Latin America. Further studies on ISVs in Ae. albopictus are needed to better understand the role of this species in the dynamics of arbovirus transmission in the Americas.
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影响因子：
3.2
作者：
通讯作者：
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使用SMART 9N扩增和纳米测序的快速病毒宏基因组学。
影响因子：
--
作者：
通讯作者：
--
影响因子：
13.2
作者：
Romo, Hannah;Kenney, Joan L.;Brault, Aaron C.
通讯作者：
Brault, Aaron C.
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ
DOI：
10.1099/vir.0.068031-0
发表时间：
2014-12
期刊：
The Journal of general virology
影响因子：
--
作者：
Kenney JL;Solberg OD;Langevin SA;Brault AC
通讯作者：
Brault AC