Therapeutic effects of Choreito, a traditional Japanese (Kampo) medicine, on detrusor overactivity induced by acetic acid in rats
Therapeutic effects of Choreito, a traditional Japanese (Kampo) medicine, on detrusor overactivity induced by acetic acid in rats
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汉方药 Choreito 对醋酸所致大鼠逼尿肌过度活动的治疗作用
DOI：
10.1111/luts.12302
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Ishizuka Osamu
中科院分区：
文献类型：
--
作者：
Ueno Manabu;Minagawa Tomonori;Saito Tetsuichi;Imamura Tetsuya;Nagai Takashi;Ogawa Teruyuki;Ishizuka Osamu
Choreito(CRT)是一种传统的日本(Kampo)药物，在日本被广泛用于治疗膀胱过度活动症(OAB)和其他下尿路症状。本研究通过建立大鼠逼尿肌过度活动(DO)模型，探讨CRT改善逼尿肌过度活动(DO)的作用及其机制。将45只雌性SD大鼠随机分为3组：生理盐水灌注组(正常组)、醋酸(AA)灌注组(AA组)和AA+CRT灌注组(AA+CRT组)。为了引起膀胱容量的减少，根据先前的研究，使用了0.2%的AA滴注。细胞计数法检测AA和CRT的作用。用激光血流仪检测微循环，免疫组织化学方法检测缺氧诱导因子1α(HIF1α)的表达。正常组、再生障碍性贫血和再生障碍性贫血合并CRT组的膀胱容量分别为1.2 ± 0.3 m L、0.4 ± 0.1 m L和0.8 ± 0.1 m L。CRT可明显减轻AA对膀胱的刺激作用，对基础压、排尿压、排尿间期和尿量均有保护作用。此外，CRT还能预防AA膀胱内灌注所致的尿路上皮下血流量过多和水肿性改变。免疫组织化学HIF1α染色各组间无明显变化。CRT可减轻AA膀胱内注射所致的DO。CRT可能通过减轻尿路上皮损伤和调节过度血流而发挥治疗OAB的作用。
Choreito (CRT), a traditional Japanese (Kampo) medicine, is widely used for the treatment of overactive bladder (OAB) and other lower urinary tract symptoms in Japan. This study aimed to identify the effects and therapeutic mechanism of CRT on the improvement of detrusor overactivity (DO) using an experimental rat model. Forty‐five female Sprague‐Dawley rats were equally divided into three groups: intravesical saline instillation with normal food (normal group), intravesical acetic acid (AA) instillation with normal food (AA group), and intravesical AA instillation with CRT (AA with CRT group). To induce a decrease in bladder capacity, instillation of 0.2% AA was used based on prior studies. Cystometric investigation was employed to clarify the effects of AA and CRT. Microcirculation was performed using a laser blood flowmeter, and the localization of hypoxia‐inducible factor 1α (HIF1α) was assessed by immunohistochemistry. The bladder capacities of the normal, AA, and AA with CRT groups were 1.2 ± 0.3 mL, 0.4 ± 0.1 mL, and 0.8 ± 0.1 mL, respectively. CRT significantly attenuated AA irritation of the urinary bladder and exerted protective effects on basal pressure, micturition pressure, micturition interval, and micturition volume. Furthermore, CRT could prevent the excess blood flow and edematous change under the urothelium induced by intravesical AA instillation. No obvious changes in immunohistochemical HIF1α staining were observed among the groups. CRT attenuated DO induced by intravesical AA instillation in a rat experimental model. CRT might impart therapeutic effects on OAB via the mitigation of urothelial damage and regulation of excess blood flow.
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日本传统草药御车神器丸对女性膀胱过度活动症的临床疗效和耐受性。
DOI：
--
发表时间：
2008
期刊：
Hinyokika kiyo. Acta urologica Japonica
影响因子：
--
作者：
M. Kajiwara;K. Mutaguchi
通讯作者：
K. Mutaguchi
影响因子：
2.2
作者：
Kono, Toru;Koseki, Takashi;Kasai, Shinichi
通讯作者：
Kasai, Shinichi
[chorei-to 和chorei-to-go-shimotsu-to 在下尿路症状患者中的临床评价]。
DOI：
--
发表时间：
1988
期刊：
Hinyokika kiyo. Acta urologica Japonica
影响因子：
--
作者：
A. Horii;M. Maekawa
通讯作者：
M. Maekawa
影响因子：
7.9
作者：
Kawashima, Nozomu;Deveaux, Teykia E.;Kato, Koji
通讯作者：
Kato, Koji
影响因子：
3.5
作者：
Gotoh, A;Goto, K;Kamidono, S
通讯作者：
Kamidono, S