An SSR from the leucine-rich repeat region of the rose Rdr1 gene family is a useful resistance gene analogue marker for roses and other Rosaceae

An SSR from the leucine-rich repeat region of the rose Rdr1 gene family is a useful resistance gene analogue marker for roses and other Rosaceae
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来自玫瑰 Rdr1 基因家族富含亮氨酸重复区域的 SSR 是玫瑰和其他蔷薇科植物的有用抗性基因类似物标记

DOI:
10.1111/j.1439-0523.2010.01780.x
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发表时间:
2010
期刊:
影响因子:
2
通讯作者:
Debener
Debener
中科院分区:
农林科学3区
文献类型:
--
作者:
Terefe;Debener

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1图1表摘要我们从9个完全测序的抗黑斑病多花蔷薇Rdr1旁系同源物的LRR区高度保守的部分开发了Rd1LRR微卫星标记。黑斑病抗性基因Rdr1属于核苷酸结合位点和富含亮氨酸重复序列(NBS‐LRR)编码基因。在每个Rdr1基因组中出现一个不同的(CT)n重复序列,使我们能够对Rdr1基因家族内的玫瑰基因型进行指纹识别。用Rd1LRR对34个玫瑰品种进行PCR分析,得到52条不同的条带,对应于至少52个不同的NBS‐LRR编码抗性基因类似物基因。此外,还对31种野生月季和34份月季种质资源进行了研究。用该标记对100个野生种进行了分析。Rd1LRR带型的数量和等位基因组成的高度多样性,即使在个人的R。结果表明,Rdr1基因座具有高度的多样性。Rd1LRR可用于未来的作图项目,用于测试新的抗性特异性对theRdr1基因簇的共分离。此外,该标记是一个有用的工具,在克隆实验中的distinctRdr1基因的鉴定。
With 1 figure and 1 tableAbstractWe developed the Rd1LRR microsatellite marker from a highly conserved part of the LRR region of nine completely sequencedRdr1paralogues of a black spot‐resistantRosa multifloragenotype. The black spot resistance geneRdr1belongs to the class of nucleotide‐binding site and leucine‐rich repeat (NBS‐LRR)‐encoding genes. The occurrence of a distinct (CT)nrepeat in eachRdr1paralogue allowed us to fingerprint rose genotypes for the diversity within theRdr1gene family. PCR analysis with Rd1LRR in 34 rose cultivars resulted in 52 distinct bands corresponding to a minimum of 52 different NBS‐LRR encoding resistance gene analogue genes. In addition, 31 wild rose species and 34 accessions ofR. arvensiswere analysed with this marker. The high diversity for the number and allelic composition of Rd1LRR banding patterns even within individuals ofR. arvensisindicated that theRdr1locus is highly diverse. The Rd1LRR may be used in future mapping projects for tests of cosegregation of new resistance specificities to theRdr1gene cluster. Furthermore, this marker is a useful tool for the identification of distinctRdr1genes in cloning experiments.
DOI: --
发表时间: 2008
期刊: Genetics
影响因子: 3.3
作者:
H. Kuang;H. V. van Eck;D. Sicard;R. Michelmore;E. Nevo
通讯作者: E. Nevo
DOI: 10.1093/nar/22.22.4673
发表时间: 1994-11-11
影响因子: 14.9
作者:
THOMPSON, JD;HIGGINS, DG;GIBSON, TJ
通讯作者: GIBSON, TJ
DOI: 10.1007/s00438-002-0784-0
发表时间: 2003-02-01
影响因子: 3.1
作者:
Kaufmann, H;Mattiesch, L;Debener, T
通讯作者: Debener, T