An SSR from the leucine-rich repeat region of the rose Rdr1 gene family is a useful resistance gene analogue marker for roses and other Rosaceae
An SSR from the leucine-rich repeat region of the rose Rdr1 gene family is a useful resistance gene analogue marker for roses and other Rosaceae
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来自玫瑰 Rdr1 基因家族富含亮氨酸重复区域的 SSR 是玫瑰和其他蔷薇科植物的有用抗性基因类似物标记
DOI:
10.1111/j.1439-0523.2010.01780.x
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发表时间:
2010
期刊:
影响因子:
2
通讯作者:
Debener
中科院分区:
文献类型:
--
作者:
Terefe;Debener
With 1 figure and 1 tableAbstractWe developed the Rd1LRR microsatellite marker from a highly conserved part of the LRR region of nine completely sequencedRdr1paralogues of a black spot‐resistantRosa multifloragenotype. The black spot resistance geneRdr1belongs to the class of nucleotide‐binding site and leucine‐rich repeat (NBS‐LRR)‐encoding genes. The occurrence of a distinct (CT)nrepeat in eachRdr1paralogue allowed us to fingerprint rose genotypes for the diversity within theRdr1gene family. PCR analysis with Rd1LRR in 34 rose cultivars resulted in 52 distinct bands corresponding to a minimum of 52 different NBS‐LRR encoding resistance gene analogue genes. In addition, 31 wild rose species and 34 accessions ofR. arvensiswere analysed with this marker. The high diversity for the number and allelic composition of Rd1LRR banding patterns even within individuals ofR. arvensisindicated that theRdr1locus is highly diverse. The Rd1LRR may be used in future mapping projects for tests of cosegregation of new resistance specificities to theRdr1gene cluster. Furthermore, this marker is a useful tool for the identification of distinctRdr1genes in cloning experiments.
影响因子:
3.3
作者:
H. Kuang;H. V. van Eck;D. Sicard;R. Michelmore;E. Nevo
通讯作者:
E. Nevo
影响因子:
14.9
作者:
THOMPSON, JD;HIGGINS, DG;GIBSON, TJ
通讯作者:
GIBSON, TJ
影响因子:
3.1
作者:
Kaufmann, H;Mattiesch, L;Debener, T
通讯作者:
Debener, T