Evolution and Genetic Population Structure of Prickly Lettuce (Lactuca serriola) and Its RGC2 Resistance Gene Cluster
Evolution and Genetic Population Structure of Prickly Lettuce (Lactuca serriola) and Its RGC2 Resistance Gene Cluster
复制标题
刺生菜及其RGC2抗性基因簇的进化和遗传群体结构
作者：
H. Kuang;H. V. van Eck;D. Sicard;R. Michelmore;E. Nevo
利用AFLP分子标记技术研究了刺莴苣（Lactuca serriola）自然居群的遗传结构和多样性，并与RGC 2抗病基因簇的多样性进行了比较。利用319个AFLP分子标记对41个居群的696份材料进行筛选，结果表明，东土耳其和亚美尼亚居群是最具多样性的居群，可能位于L. serriola。利用位于大多数RGC 2同源基因编码区的微卫星MSATE 6对709份材料进行筛选，检测到366种不同的单倍型。同样，东部土耳其和亚美尼亚人口在RGC 2集群中的差异最大。不同群体RGC 2簇的遗传多样性与其全基因组遗传多样性显著相关。RGC 2同源基因拷贝数在不同群体中存在显著差异，每个基因组拷贝数在12 - 22个之间。RGC 2基因（K和L）的两个保守谱系（II型）的核苷酸差异与使用MSATE 6或AFLP数据计算的差异不相关。我们推测，高基因组多样性和多样性的RGC 2集群在东部土耳其和亚美尼亚人口造成的高非生物和生物胁迫的地区原产地L。serriola。
Genetic structure and diversity of natural populations of prickly lettuce (Lactuca serriola) were studied using AFLP markers and then compared with the diversity of the RGC2 disease resistance gene cluster. Screening of 696 accessions from 41 populations using 319 AFLP markers showed that eastern Turkish and Armenian populations were the most diverse populations and might be located in the origin and center of diversity of L. serriola. Screening 709 accessions using the microsatellite MSATE6 that is located in the coding region of most RGC2 homologs detected 366 different haplotypes. Again, the eastern Turkish and Armenian populations had the highest diversities at the RGC2 cluster. The diversities at the RGC2 cluster in different populations were significantly correlated with their genomewide diversities. There was significant variation of copy number of RGC2 homologs in different populations, ranging from 12 to 22 copies per genome. The nucleotide diversities of two conserved lineages (type II) of RGC2 genes (K and L) were not correlated with diversities calculated using the MSATE6 or AFLP data. We hypothesize that the high genomewide diversity and diversity of the RGC2 cluster in eastern Turkish and Armenian populations resulted from high abiotic and biotic stresses in the regions of origin of L. serriola.
DOI：
10.1073/pnas.96.1.302
发表时间：
1999-01-05
影响因子：
11.1
作者：
Caicedo, AL;Schaal, BA;Kunkel, BN
通讯作者：
Kunkel, BN
影响因子：
3.3
作者：
Yunxin Fu
通讯作者：
Yunxin Fu
影响因子：
3.3
作者：
R. Mauricio;E. Stahl;T. Korves;Dacheng Tian;M. Kreitman;J. Bergelson
通讯作者：
R. Mauricio;E. Stahl;T. Korves;Dacheng Tian;M. Kreitman;J. Bergelson