Role of kinesin heavy chain in Crumbs localization along the rhabdomere elongation in Drosophila photoreceptor.
Role of kinesin heavy chain in Crumbs localization along the rhabdomere elongation in Drosophila photoreceptor.
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DOI：
10.1371/journal.pone.0021218
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Nam SC
中科院分区：
文献类型：
--
作者：
League GP;Nam SC
Crumbs (Crb) 是一种细胞极性基因，已被证明可以为果蝇光感受器形态发生过程中顶膜结构域、粘附连接 (AJ) 和横纹肌沿生长的近端-远端轴的延伸提供位置提示。在果蝇光感受器的发育过程中，发现了稳定的微管结构，其存在与极性蛋白定位有关。因此推测微管可能在光感受器形态发生过程中为极性蛋白提供运输途径。这项研究检验了驱动蛋白重链 (Khc)（基于微管的运动驱动蛋白-1 的一个亚基）是否对于发育中的光感受器极性蛋白定位至关重要。由于在 crb 和 khc 之间发现了遗传相互作用，因此在 khc 突变体的发育中的光感受器中检查了 Crb 的定位。 khc 在早期眼睛分化和发育过程中是可有可无的。然而，khc 突变型光感受器在顶膜域中表现出一系列异常，具体取决于蛹光感受器沿近端-远端轴的位置。 khc突变体在横纹肌伸长过程中沿着远近轴在顶端区域表现出渐进性错位。 khc 突变也导致稳定的微管结构出现类似的进行性缺陷，强烈表明 Khc 对于蛹形态发生中从远端到近端横纹肌伸长过程中的微管结构和 Crb 定位至关重要。 Khc 的功能获得表型进一步支持了 Khc 在顶端域控制中的作用。 Khc 在光感受器中的过度表达导致顶膜结构域和发育中的光感受器中稳定的微管的破坏。总之，我们研究了 khc 在光感受器发育过程中顶端 Crb 结构域的调节中的作用。由于发育中的蛹眼中的横纹小球沿着远端-近端轴生长，这些表型表明Khc对于光感受器远端-近端伸长过程中的微管结构和顶膜域至关重要，但对于早期眼睛发育来说是可有可无的。
Crumbs (Crb), a cell polarity gene, has been shown to provide a positional cue for the extension of the apical membrane domain, adherens junction (AJ), and rhabdomere along the growing proximal-distal axis during Drosophila photoreceptor morphogenesis. In developing Drosophila photoreceptors, a stabilized microtubule structure was discovered and its presence was linked to polarity protein localization. It was therefore hypothesized that the microtubules may provide trafficking routes for the polarity proteins during photoreceptor morphogenesis. This study has examined whether Kinesin heavy chain (Khc), a subunit of the microtubule-based motor Kinesin-1, is essential in polarity protein localization in developing photoreceptors. Because a genetic interaction was found between crb and khc, Crb localization was examined in the developing photoreceptors of khc mutants. khc was dispensable during early eye differentiation and development. However, khc mutant photoreceptors showed a range of abnormalities in the apical membrane domain depending on the position along the proximal-distal axis in pupal photoreceptors. The khc mutant showed a progressive mislocalization in the apical domain along the distal-proximal axis during rhabdomere elongation. The khc mutation also led to a similar progressive defect in the stabilized microtubule structures, strongly suggesting that Khc is essential for microtubule structure and Crb localization during distal to proximal rhabdomere elongation in pupal morphogenesis. This role of Khc in apical domain control was further supported by khc's gain-of-function phenotype. Khc overexpression in photoreceptors caused disruption of the apical membrane domain and the stabilized microtubules in the developing photoreceptors. In summary, we examined the role of khc in the regulation of the apical Crb domain in developing photoreceptors. Since the rhabdomeres in developing pupal eyes grow along the distal-proximal axis, these phenotypes suggest that Khc is essential for the microtubule structures and apical membrane domains during the distal-proximal elongation of photoreceptors, but is dispensable for early eye development.
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DOI：
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影响因子：
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