Protective immunity to HIV-1 in SCID/beige mice reconstituted with peripheral blood lymphocytes of exposed but uninfected individuals.
Protective immunity to HIV-1 in SCID/beige mice reconstituted with peripheral blood lymphocytes of exposed but uninfected individuals.
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用暴露但未感染个体的外周血淋巴细胞重建 SCID/米色小鼠对 HIV-1 的保护性免疫力。
DOI：
--
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发表时间：
1996
影响因子：
11.1
通讯作者：
Lung Ji Chang
中科院分区：
文献类型：
--
作者：
Chengsheng Zhang;Yan Cui;S. Houston;Lung Ji Chang
Immunodeficiency typically appears many years after initial HIV infection. This long, essentially asymptomatic period contributes to the transmission of HIV in human populations. In rare instances, clearance of HIV-1 infection has been observed, particularly in infants. There are also reports of individuals who have been frequently exposed to HIV-1 but remain seronegative for the virus, and it has been hypothesized that these individuals are resistant to infection by HIV-1. However, little is known about the mechanism of immune clearance or protection against HIV-1 in these high-risk individuals because it is difficult to directly demonstrate in vivo protective immunity. Although most of these high-risk individuals show an HIV-1-specific cell-mediated immune response using in vitro assays, their peripheral blood lymphocytes (PBLs) are still susceptible to HIV infection in tissue culture. To study this further in vivo, we have established a humanized SCID mouse infection model whereby T-, B-, and natural killer-cell defective SCID/beige mice that have been reconstituted with normal human PBLs can be infected with HIV-1. When the SCID/beige mice were reconstituted with PBLs from two different multiply exposed HIV-1 seronegative individuals, the mice showed resistance to infection by two strains of HIV-1 (macrophage tropic and T cell tropic), although the same PBLs were easily infected in vitro. Mice reconstituted with PBLs from non-HIV-exposed controls were readily infected. When the same reconstituted mice were depleted of human CD8 T cells, however, they became susceptible to HIV-1 infection, indicating that the in vivo protection required CD8 T cells. This provides clear experimental evidence that some multiply exposed, HIV-1-negative individuals have in vivo protective immunity that is CD8 T cell-dependent. Understanding the mechanism of such protective immunity is critical to the design and testing of effective prophylactic vaccines and immunotherapeutic regimens.
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影响因子：
158.5
作者：
HO, DD;MOUDGIL, T;ALAM, M
通讯作者：
ALAM, M
高危 HIV-1 血清阴性血友病患者中人类免疫缺陷病毒 (HIV) 1 型感染状况和体外对 HIV 感染的易感性。
DOI：
10.1093/infdis/172.1.228
发表时间：
1995
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Lederman,MM;Jackson,JB;Kroner,BL;White3rd,GC;Eyster,ME;Aledort,LM;Hilgartner,MW;Kessler,CM;Cohen,AR;Kiger,KP
通讯作者：
Kiger,KP
影响因子：
3.8
作者：
J. Safrit;M. Fung;C. Andrews;D. Braun;W. Sun;T. Chang;R. Koup
通讯作者：
J. Safrit;M. Fung;C. Andrews;D. Braun;W. Sun;T. Chang;R. Koup
用来自接种牛痘 gp160 和重组 gp160 的捐赠者的外周血白细胞重建的 SCID 小鼠对人类免疫缺陷病毒 1 感染的抵抗力。
DOI：
10.1073/pnas.90.6.2443
发表时间：
1993
影响因子：
11.1
作者：
Mosier,DE;Gulizia,RJ;MacIsaac,PD;Corey,L;Greenberg,PD
通讯作者：
Greenberg,PD
影响因子：
6.4
作者：
CLERICI, M;GIORGI, JV;SHEARER, GM
通讯作者：
SHEARER, GM