Design and Validation of a Short Novel Estradiol Aptamer and Exploration of Its Application in Sensor Technology.
Design and Validation of a Short Novel Estradiol Aptamer and Exploration of Its Application in Sensor Technology.
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DOI：
10.3390/molecules29020535
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发表时间：
2024-01-22
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17β-雌二醇 (E2) 的特异性和灵敏检测对于诊断和治疗多种疾病至关重要，适配体已成为开发检测平台的有前途的识别探针。然而，传统的长序列E2适体由于冗余结构影响其稳定性和识别能力而表现出有限的临床性能。因此迫切需要进一步优化适配体结构，构建有效的E2检测平台。在这项工作中，我们设计了一种新型的短适体，它保留了传统适体与E2的关键结合结构，同时消除了多余的结构。使用微尺度热泳、基于金纳米颗粒的比色法和电化学测定来评估所提出的适体的结合特性。我们的结果表明，所提出的适配体对E2具有优异的特异性识别能力和解离常数为92 nM的高亲和力。此外，该适配体作为识别探针在构建高度特异性和灵敏的临床雌二醇检测平台方面显示出巨大的潜力。基于适配体的电化学传感器能够在5 pg mL–1和10 ng mL–1（R2 = 0.973）之间线性范围内检测E2，并且一定低浓度水平的检测能力为5 pg mL–1（S/N = 3）。总体而言，这种新型适体作为未来研究 E2 在各种生理和病理过程中的作用以及开发临床应用中 E2 检测的敏感和特异诊断测定的有价值的工具具有广阔的前景。
The specific and sensitive detection of 17β-estradiol (E2) is critical for diagnosing and treating numerous diseases, and aptamers have emerged as promising recognition probes for developing detection platforms. However, traditional long-sequence E2 aptamers have demonstrated limited clinical performance due to redundant structures that can affect their stability and recognition ability. There is thus an urgent need to further optimize the structure of the aptamer to build an effective detection platform for E2. In this work, we have designed a novel short aptamer that retains the key binding structure of traditional aptamers to E2 while eliminating the redundant structures. The proposed aptamer was evaluated for its binding properties using microscale thermophoresis, a gold nanoparticle-based colorimetric method, and electrochemical assays. Our results demonstrate that the proposed aptamer has excellent specific recognition ability for E2 and a high affinity with a dissociation constant of 92 nM. Moreover, the aptamer shows great potential as a recognition probe for constructing a highly specific and sensitive clinical estradiol detection platform. The aptamer-based electrochemical sensor enabled the detection of E2 with a linear range between 5 pg mL–1 and 10 ng mL–1 (R2 = 0.973), and the detection capability of a definite low concentration level was 5 pg mL–1 (S/N = 3). Overall, this novel aptamer holds great promise as a valuable tool for future studies on the role of E2 in various physiological and pathological processes and for developing sensitive and specific diagnostic assays for E2 detection in clinical applications.
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影响因子：
12.6
作者：
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影响因子：
8.8
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影响因子：
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DOI：
10.1073/pnas.85.21.7942
发表时间：
1988-11-01
影响因子：
11.1
作者：
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通讯作者：
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折叠式纸基适配体传感器平台涂有新型纳米组件，可即时、高灵敏地检测 17β-雌二醇
影响因子：
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作者：
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通讯作者：
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