Changes in Ovulation-Related Gene Expression during Induced Ovulation in the Amur Sturgeon (Acipenser schrenckii) Ovarian Follicles.
Changes in Ovulation-Related Gene Expression during Induced Ovulation in the Amur Sturgeon (Acipenser schrenckii) Ovarian Follicles.
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DOI：
10.3390/ijms232113143
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发表时间：
2022-10-29
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
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促黄体生成素（LH）和成熟诱导类固醇（MIS）如17α，20 β-二羟基-4-异戊烯-3-酮（17α，20 β-dihydroxy-4-dichloren-3-one）调节硬骨鱼类卵母细胞的最终成熟。卵母细胞成熟能力（OMC）和排卵能力分别衡量卵母细胞成熟和排卵对MIS的敏感性。然而，获得排卵能力的分子机制仍不清楚。鲟鱼是研究这些机制的一个很好的研究模型。我们研究了体外OMC和排卵能力的季节性分布，并采用定量PCR技术检测了17个排卵相关候选基因在施氏鲟卵泡中的表达。获得OMC后，通过LH释放激素类似物（LHRHa）引发注射诱导排卵能力，其在春季或秋季自发诱导。在从无排卵和排卵鱼中取样的卵巢滤泡中注射LHRHa引发后，包括组织型纤溶酶原激活剂（plat）在内的七个基因得到增强。激活素受体1型（acvr 1）和前列腺素G/H合酶2（ptgs 2）仅在排卵的鱼中上调。我们的研究结果表明，plat/纤溶酶和前列腺素（PG）/PG受体系统是必不可少的鲟鱼排卵，类似于其他脊椎动物。值得注意的是，成功的排卵取决于足够的PG合成，激活PG/PG受体系统的介质是获得排卵能力所必需的。我们提供了第一个报告的排卵相关基因的改变，在卵巢卵泡的阿穆尔鲟鱼。
The luteinizing hormone (LH) and maturation-inducing steroids (MIS), such as 17α,20β-dihydroxy-4-pregnen-3-one, regulate the final oocyte maturation in teleosts. Oocyte maturational competence (OMC) and ovulatory competence measure the sensitivity to MIS for oocyte maturation and ovulation, respectively. However, the molecular mechanisms underlying the acquisition of ovulatory competence remain unknown. Sturgeons are an excellent research model for investigating these mechanisms. We examined the seasonal profiles of OMC and ovulatory competence in vitro and the expression of 17 ovulation-related gene candidates using quantitative PCR in Amur sturgeon ovarian follicles. The ovulatory competence was induced by the LH-releasing hormone analog (LHRHa) priming injection after acquiring the OMC, which was spontaneously induced in spring or autumn. Seven genes, including the tissue-type plasminogen activator (plat), were enhanced following the LHRHa priming injection in ovarian follicles sampled from anovulated and ovulated fish. The activin receptor type 1 (acvr1) and prostaglandin G/H synthase 2 (ptgs2) were only upregulated in ovulated fish. Our results suggest that plat/plasmin and prostaglandin (PG)/PG receptor systems are essential for sturgeon ovulation, similar to other vertebrates. Notably, successful ovulation depends on a sufficient PG synthesis, and mediators activating the PG/PG receptor system are essential for acquiring the ovulatory competence. We provide the first report of ovulation-related gene alterations in the ovarian follicles of Amur sturgeons.
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影响因子：
4.6
作者：
Crespo D;Goetz FW;Planas JV
通讯作者：
Planas JV
马卵泡成熟过程中细胞分泌的外泌体对 ACVR1 和 ID2 的调节。
DOI：
10.1186/1477-7827-12-44
发表时间：
2014-05-26
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
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通讯作者：
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作者：
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DOI：
10.1051/rnd:19780534
发表时间：
1978-01-01
期刊：
ANNALES DE BIOLOGIE ANIMALE BIOCHIMIE BIOPHYSIQUE
影响因子：
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作者：
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通讯作者：
FOSTIER, A
影响因子：
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作者：
Fujimori, Chika;Ogiwara, Katsueki;Takahashi, Takayuki
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Takahashi, Takayuki