Single cell transcriptomics of hypothalamic warm sensitive neurons that control core body temperature and fever response Signaling asymmetry and an extension of chemical neuroanatomy.
Single cell transcriptomics of hypothalamic warm sensitive neurons that control core body temperature and fever response Signaling asymmetry and an extension of chemical neuroanatomy.
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DOI：
10.1016/j.pharmthera.2010.09.010
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发表时间：
2011-03
影响因子：
13.5
通讯作者：
Bartfai, Tamas
中科院分区：
文献类型：
--
作者：
Eberwine, James;Bartfai, Tamas
关键词：
我们报道了通过建立每个个体的、电生理识别的温敏神经元(WSN)的cDNA文库，对活跃在中央下丘脑前部神经元回路中的单个成年神经元进行“无偏见”的分子表征。用Affymetrix微阵列对文库进行分析。通过对每个单细胞文库进行Illumina测序，证实并增强了cDNA的存在和频率。编码GABA生物合成酶的cDNA。GAD1和肾上腺髓质素、甘丙肽、前强啡肽、生长抑素和速激肽在WSNs中均有表达。对500多个神经递质受体、激素受体和离子通道的功能细胞和体内研究表明，在WSNs中，转录和功能数据很少或没有差异；只要对其cDNA被鉴定的受体有激动剂，就会发现功能反应。通过对单个神经元文库进行测序，可以识别胰岛素受体、脂联素受体2和受体异二聚体等很少表达的受体；当混合细胞导致信号稀释和混合信号时，丢失的信息。尽管有共同的电生理表型和统一的GAD1表达，但WSN-转录组显示出异质性，表明转录组受到强烈的表观遗传影响。我们的研究表明，通过测序和芯片来查询单个神经元的cDNA文库是很有价值的。
We report on an ‘unbiased’ molecular characterization of individual, adult neurons, active in a central, anterior hypothalamic neuronal circuit, by establishing cDNA libraries from each individual, electrophysiologically identified warm sensitive neuron (WSN). The cDNA libraries were analyzed by Affymetrix microarray. The presence and frequency of cDNAs was confirmed and enhanced with Illumina sequencing of each single cell cDNA library. cDNAs encoding the GABA biosynthetic enzyme. GAD1 and of adrenomedullin, galanin, prodynorphin, somatostatin, and tachykinin were found in the WSNs. The functional cellular and in vivo studies on dozens of the more than 500 neurotransmitter -, hormone- receptors and ion channels, whose cDNA was identified and sequence confirmed, suggest little or no discrepancy between the transcriptional and functional data in WSNs; whenever agonists were available for a receptor whose cDNA was identified, a functional response was found.. Sequencing single neuron libraries permitted identification of rarely expressed receptors like the insulin receptor, adiponectin receptor2 and of receptor heterodimers; information that is lost when pooling cells leads to dilution of signals and mixing signals. Despite the common electrophysiological phenotype and uniform GAD1 expression, WSN- transcriptomes show heterogenity, suggesting strong epigenetic influence on the transcriptome. Our study suggests that it is well-worth interrogating the cDNA libraries of single neurons by sequencing and chipping.
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DOI：
10.1085/jgp.50.6.1469
发表时间：
1967-07
期刊：
The Journal of general physiology
影响因子：
--
作者：
Carpenter DO
通讯作者：
Carpenter DO
影响因子：
5.5
作者：
BALDINO, F;GELLER, HM
通讯作者：
GELLER, HM
影响因子：
--
作者：
DEAN, JB;BOULANT, JA
通讯作者：
BOULANT, JA
影响因子：
56.9
作者：
Conti, Bruno;Sanchez-Alavez, Manuel;Bartfai, Tamas
通讯作者：
Bartfai, Tamas
DOI：
10.1111/j.1749-6632.1998.tb08315.x
发表时间：
1998-01-01
期刊：
MOLECULAR MECHANISMS OF FEVER
影响因子：
--
作者：
Coceani, F;Akarsu, ES
通讯作者：
Akarsu, ES