Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video).
Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video).
复制标题
使用共聚焦激光内镜对结直肠癌中的 Ulex Europaeus 凝集素进行分子成像（附视频）
DOI：
10.3389/fonc.2021.792420
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发表时间：
2021
影响因子：
4.7
通讯作者：
Lin R
中科院分区：
文献类型：
--
作者：
Wang W;Tian S;Jiang X;Pang S;Shi H;Fan M;Wang Z;Jiang W;Hu W;Xiao X;Lin R
背景和研究目的以前的研究已经确定结直肠癌与正常结肠相比具有不同的岩藻糖基化水平。荆豆凝集素-I（UEA-I）与α1-2岩藻糖聚糖特异性结合，常用于检测岩藻糖基化水平。因此，我们使用共聚焦激光显微内镜（CLE），以探讨荧光标记的UEA-异硫氰酸荧光素（FITC）检测colonic cancer.Patients和MethodsWe染色冷冻小鼠结肠组织切片正常，腺瘤，腺癌物种与UEA-FITC检测岩藻糖基化水平在不同的群体。首先使用白色光内镜和细胞内镜检查病变。然后在体外对小鼠和人结肠组织进行UEA-FITC染色。用CLE检测相应病变的UEA-FITC水平，并记录视频进行定量分析。UEA-FITC使用CLE的诊断准确性进行了评价的敏感性和特异性。ResultsUEA在大肠癌的表达水平低于正常肠上皮。CLE检测到的人和小鼠大肠癌组织中UEA-FITC的荧光强度比均显著低于正常组织。UEA-FITC与CLE联合检测大肠癌的敏感性为95.6%，特异性为97.7%。阳性和阴性预测值分别为91.6%和95.6%。总体而言，95.6%的网站被正确分类CLE.ConclusionsWe开发了一种新的成像策略，以提高CLE的诊断效率，通过使用UEA-FITC。
Background and Study AimsPrevious studies have identified that colorectal cancer has different fucosylation levels compared to the normal colon. Ulex europaeus agglutinin-I (UEA-I), which specifically combines with α1-2 fucose glycan, is usually used to detect fucosylation levels. Therefore, we used confocal laser endomicroscopy (CLE) to investigate fluorescently labeled UEA-Fluorescein isothiocyanate (FITC) for detecting colonic cancer.Patients and MethodsWe stained frozen mouse colon tissue sections of normal, adenoma, and adenocarcinoma species with UEA-FITC to detect fucosylation levels in different groups. White light endoscopy and endocytoscopy were first used to detect the lesions. The UEA-FITC was then stained in the mice and human colon tissuesin vitro. The CLE was used to detect the UEA-FITC levels of the corresponding lesions, and videos were recorded for quantitation analysis. The diagnostic accuracy of UEA-FITC using CLE was evaluated in terms of sensitivity and specificity.ResultsThe UEA expression level in colorectal cancer was lower than that in normal intestinal epithelium. The fluorescence intensity ratio of UEA-FITC in colorectal cancer was significantly lower than that in normal tissue detected by CLE in both mice and humans. The combination of UEA-FITC and CLE presented a good diagnostic accuracy with a sensitivity of 95.6% and a specificity of 97.7% for detecting colorectal cancer. The positive and negative predictive values were 91.6% and 95.6%, respectively. Overall, 95.6% of the sites were correctly classified by CLE.ConclusionsWe developed a new imaging strategy to improve the diagnostic efficacy of CLE by using UEA-FITC.
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影响因子：
29.4
作者：
Wang Y;Huang D;Chen KY;Cui M;Wang W;Huang X;Awadellah A;Li Q;Friedman A;Xin WW;Di Martino L;Cominelli F;Miron A;Chan R;Fox JG;Xu Y;Shen X;Kalady MF;Markowitz S;Maillard I;Lowe JB;Xin W;Zhou L
通讯作者：
Zhou L
影响因子：
16.6
作者：
Isermann T;Şener ÖÇ;Stender A;Klemke L;Winkler N;Neesse A;Li J;Wegwitz F;Moll UM;Schulz-Heddergott R
通讯作者：
Schulz-Heddergott R
DOI：
10.1111/den.12164
发表时间：
2014-05
期刊：
Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society
影响因子：
--
作者：
Ichimasa K;Kudo SE;Mori Y;Wakamura K;Ikehara N;Kutsukawa M;Takeda K;Misawa M;Kudo T;Miyachi H;Yamamura F;Ohkoshi S;Hamatani S;Inoue H
通讯作者：
Inoue H
用于监测溃疡性结肠炎的内窥镜三模态成像：高分辨率内窥镜和自发荧光成像对肿瘤检测的随机比较；以及用于病变分类的窄带成像评估。
影响因子：
24.5
作者：
van den Broek, F. J. C.;Fockens, P.;van Eeden, S.;Reitsma, J. B.;Hardwick, J. C. H.;Stokkers, P. C. F.;Dekker, E.
通讯作者：
Dekker, E.
影响因子：
29.4
作者：
van der Sommen, Fons;Curvers, Wouter L.;Nagengast, Wouter B.
通讯作者：
Nagengast, Wouter B.