Transmembrane redistribution of phospholipids of the human red cell membrane during hypotonic hemolysis.
Transmembrane redistribution of phospholipids of the human red cell membrane during hypotonic hemolysis.
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低渗溶血过程中人红细胞膜磷脂的跨膜重新分布。
DOI：
10.1016/0005-2736(92)90176-m
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Devaux,PF
中科院分区：
文献类型：
--
作者：
Schrier,SL;Zachowski,A;Hervé,P;Kader,JC;Devaux,PF
用电子顺磁共振法测定了低渗溶血前后红细胞中自旋标记磷脂的跨膜分布。当细胞在没有Mg-ATP的情况下被重新密封，或者当氨基磷脂转位酶被钒酸盐或钙抑制时，用第一批部分水溶性探针实现了磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸和鞘霉素类似物的完全随机化。如果用Mg-ATP内部重新密封，则氨基磷脂的跨膜不对称重新建立。对于长链不溶性自旋标记脂类，磷脂酰胆碱类似物获得了完全随机化，但即使在钒酸盐存在时，也只有很小的百分比（约为10%）。15%)的自旋标记的磷脂酰丝氨酸翻转到外层，而相当比例的自旋标记的鞘肌素翻转到内层，这表明在溶血过程中这些不溶性脂质的磷脂再分配存在层次。选择性随机化发生的机制尚不清楚。它可能包括内小叶磷脂酰丝氨酸-蛋白质相互作用和外小叶鞘磷脂-胆固醇或鞘磷脂-鞘磷脂相互作用。
The transmembrane distribution of spin-labeled phospholipids was measured in human erythrocytes before and after hypotonic hemolysis by electron paramagnetic resonance. With a first series of partially water soluble probes a complete randomization of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and sphingomyclin analogues was achieved when cells were resealed in the absence of Mg-ATP or when the aminophospholipid translocase was inhibited by vanadate or calcium. If the ghosts were resealed with Mg-ATP inside, the transmembrane asymmetry of the aminophospholipids was reestablished. With long chain insoluble spin-labeled lipids complete randomization was obtained with the phosphatidylcholine analogue but even in the presence of vanadate only a small percentage (approx. 15%) of the spin-labeled phosphatidylserine flopped to the outer monolayer and comparable percentage of the spin-labeled sphingomyclin flipped to the inner monolayer, indicating a hierarchy in the phospholipid redistribution for these water insoluble lipids during hemolysis. The mechanism by which a selective randomization takes place is not known. It may involve phosphatidylserine-protein interactions in the inner leaflet and sphingomyelin-cholesterol or sphingomyelin-sphingomyelin interaction in the outer leaflet.
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血影蛋白参与维持红细胞膜相态不对称性
影响因子：
64.5
作者：
P. Williamson;J. Bateman;K. Kozarsky;K. Mattocks;Neal Hermanowicz;Hye‐Ryun Choe;R. Schlegel
通讯作者：
R. Schlegel
影响因子：
2.9
作者：
M. Sankaram;T. E. Thompson
通讯作者：
M. Sankaram;T. E. Thompson
DOI：
10.1016/0005-2736(84)90170-6
发表时间：
1984-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
DRESSLER, V;HAEST, CWM;ERUSALIMSKY, JD
通讯作者：
ERUSALIMSKY, JD
影响因子：
3.4
作者：
M. Colleau;P. Hervé;P. Fellmann;P. Devaux
通讯作者：
M. Colleau;P. Hervé;P. Fellmann;P. Devaux
氨基磷脂跨人红细胞膜的 ATP 依赖性易位
影响因子：
3.5
作者：
L. Tilley;S. Cribier;B. Roelofsen;J. den Kamp;L. van Deenen
通讯作者：
L. van Deenen