Earlier and More Severe Cartilage Degeneration Occurs After Meniscal Tears in Juvenile Rabbits Compared with Adults.
Earlier and More Severe Cartilage Degeneration Occurs After Meniscal Tears in Juvenile Rabbits Compared with Adults.
复制标题
与成年兔相比，幼年兔半月板撕裂后出现更早、更严重的软骨退化
DOI：
10.1177/19476035221138959
复制
发表时间：
2023-03
期刊：
影响因子：
2.8
通讯作者：
Ao, Yingfang
中科院分区：
文献类型：
--
作者：
Yan, Wenqiang;Fan, Yifei;Dai, Wenli;Cheng, Jin;Zhao, Fengyuan;Yang, Shuai;Li, Yuwan;Maimaitimin, Maihemuti;Cao, Chenxi;Shao, Zhenxing;Li, Zong;Wang, Hongde;Hu, Xiaoqing;Ao, Yingfang
目的比较幼兔和成年兔髋臼撕裂后软骨退变的严重程度。实验设计：幼兔20只（出生后2周），成年兔20只（出生后6个月）。在右膝内侧半月板前角制备半月板撕裂。分别于术后1、3、6、12周处死动物。对股骨内侧髁和胫骨内侧平台的软骨退化进行了肉眼和组织学评价。软骨退变的半定量评估采用宏观Outerbridge评分系统和组织学OARSI评分系统。结果幼年兔术后1周软骨形态完整，细胞外基质结构正常。术后3周组织学检查可见股骨内侧髁软骨表面轻度凹凸不平，甲苯胺蓝耗竭。术后6周和12周观察到软骨恶化，表现为软骨纤维化、显著的细胞死亡和糖胺聚糖（GAG）释放。在成年兔中，术后12周仅在股骨内侧髁观察到轻度软骨退变。Outerbridge和OARSI评分的结果与幼龄和成年家兔的上述结果一致。结论幼年兔实验性关节软骨撕裂后软骨退变较成年兔更早、更严重。此外，撕裂后软骨退变表现出区域特异性，与撕裂位置相对应。然而，在将动物模型的结果外推到人类时，需要谨慎。
Objective To compare the severity of cartilage degeneration after meniscal tears between juvenile and adult rabbits. Design This study included 20 juvenile rabbits (2 weeks after birth) and 20 adult rabbits (6 months after birth). Meniscal tears were prepared in the anterior horn of medial menisci of right knees. Rabbits were sacrificed at 1, 3, 6, and 12 weeks postoperatively. Cartilage degenerations in the medial femoral condyle and medial tibial plateau were evaluated macroscopically and histologically. The semiquantitative assessment of cartilage degeneration was graded by macroscopic Outerbridge scoring system and histological Osteoarthritis Research Society International (OARSI) scoring system. Results In juvenile rabbits, the morphologically intact cartilage and normal extracellular matrix architecture were observed at the first week postoperatively. Mild uneven cartilage surface and toluidine blue depletion in the medial femoral condyle were observed on histological assessment at 3 weeks postoperatively. The worsened cartilage deterioration demonstrating chondral fibrillation, prominent cell death, and glycosaminoglycan (GAG) release was observed at 6 and 12 weeks postoperatively. In adult rabbits, only mild cartilage degeneration was observed in the medial femoral condyle at 12 weeks postoperatively. The outcomes of Outerbridge and OARSI scores were consistent with the aforementioned findings in juvenile and adult rabbits. Conclusions Our study validated that earlier and more severe cartilage degenerations were observed in juvenile rabbits after meniscal tears compared with adult rabbits. Moreover, the post-tear cartilage degeneration demonstrated regional specificity corresponded to the tear position. However, caution is warranted when extrapolating results of animal models to humans.
登录
查看更多内容
影响因子：
6.1
作者：
Logerstedt, David S.;Scalzitti, David A.;McDonough, Christine M.
通讯作者：
McDonough, Christine M.
影响因子：
5.3
作者：
Kim, Sunny;Bosque, Jose;Marder, Richard
通讯作者：
Marder, Richard
影响因子：
7
作者：
Patwari, P;Gaschen, V;Hunziker, EB
通讯作者：
Hunziker, EB
影响因子：
7
作者：
Bagi, C. M.;Berryman, E. R.;Lane, N. E.
通讯作者：
Lane, N. E.
影响因子：
2.8
作者：
Quinn, TM;Allen, RG;Hunziker, EB
通讯作者：
Hunziker, EB