Involvement of selective epithelial cell death in the formation of feather buds on a bioengineered skin
Involvement of selective epithelial cell death in the formation of feather buds on a bioengineered skin
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选择性上皮细胞死亡参与生物工程皮肤上羽毛芽的形成
DOI：
10.1111/dgd.12593
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Mitsui Toshiyuki
中科院分区：
文献类型：
--
作者：
Ishida Kentaro;Saito Tetsuichiro;Mitsui Toshiyuki
细胞凋亡导致的选择性细胞死亡在器官发生中起着重要作用。在一些外胚层器官如毛发、羽毛和乳腺的发育和稳态过程中观察到凋亡细胞。在鸡羽毛发育过程中，在羽毛形态发生过程中观察到细胞凋亡事件，但在早期羽毛芽形成过程中尚未研究。之前，我们已经报道了一种在三维培养中从解离的皮肤上皮细胞和间充质细胞在生物工程皮肤上产生羽芽的方法。在生物工程皮肤的发育过程中，在上皮组织中观察到细胞凋亡导致的上皮腔形成。在这项研究中，我们研究了选择性上皮细胞死亡过程中的生物工程皮肤的发展。组织学分析表明，生物工程皮肤中的选择性上皮细胞死亡是由caspase-3相关的细胞凋亡诱导的。用泛半胱天冬酶抑制剂处理扰乱了生物工程皮肤的羽芽形成。泛半胱天冬酶抑制剂处理抑制了上皮层的重排和真皮冷凝的形成，这被认为是形成羽芽的必要步骤。通过添加GSK-3β抑制剂（其激活Wnt/β-连环蛋白信号传导），对泛胱天蛋白酶抑制剂处理的皮肤上羽芽形成的抑制得到部分补偿。这些结果表明，上皮细胞死亡参与了生物工程皮肤的羽芽形成。这些观察结果还表明，半胱天冬酶活性和Wnt/β-连环蛋白信号传导可能有助于生物工程皮肤中上皮和间充质组分的形成。
Selective cell death by apoptosis plays important roles in organogenesis. Apoptotic cells are observed in the developmental and homeostatic processes of several ectodermal organs, such as hairs, feathers, and mammary glands. In chick feather development, apoptotic events have been observed during feather morphogenesis, but have not been investigated during early feather bud formation. Previously, we have reported a method for generating feather buds on a bioengineered skin from dissociated skin epithelial and mesenchymal cells in three‐dimensional culture. During the development of the bioengineered skin, epithelial cavity formation by apoptosis was observed in the epithelial tissue. In this study, we examined the selective epithelial cell death during the bioengineered skin development. Histological analyses suggest that the selective epithelial cell death in the bioengineered skin was induced by caspase‐3‐related apoptosis. The formation of feather buds of the bioengineered skin was disturbed by the treatment with a pan‐caspase inhibitor. The pan‐caspase inhibitor treatment suppressed the rearrangement of the epithelial layer and the formation of dermal condensation, which are thought to be essential step to form feather buds. The suppression of the formation of feather buds on the pan‐caspase inhibitor‐treated skin was partially compensated by the addition of a GSK‐3β inhibitor, which activates Wnt/β‐catenin signaling. These results suggest that the epithelial cell death is involved in the formation of feather buds of the bioengineered skin. These observations also suggest that caspase activities and Wnt/β‐catenin signaling may contribute to the formation of epithelial and mesenchymal components in the bioengineered skin.
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皮肤的形态发生
DOI：
--
发表时间：
1977
期刊：
The Yale Journal of Biology and Medicine
影响因子：
--
作者：
J. Nordlund
通讯作者：
J. Nordlund
影响因子：
6.5
作者：
Chang, CH;Yu, MK;Chuong, CM
通讯作者：
Chuong, CM
周皮紧密连接的分子结构与表皮闭塞斑的分子结构不同。
影响因子：
6.5
作者：
K. Morita;M. Furuse;Y. Yoshida;M. Itoh;H. Sasaki;S. Tsukita;Y. Miyachi
通讯作者：
Y. Miyachi
DOI：
10.1073/pnas.0811848106
发表时间：
2009-02-03
影响因子：
11.1
作者：
Wang, Weidong;Walker, John R.;Schultz, Peter G.
通讯作者：
Schultz, Peter G.
纯合基因缺陷揭示了半胱天冬酶在细胞凋亡、发育和细胞因子成熟中的作用。
影响因子：
4
作者：
Wang,J;Lenardo,MJ
通讯作者：
Lenardo,MJ