Cooperative adaptation to establishment of a synthetic bacterial mutualism.
Cooperative adaptation to establishment of a synthetic bacterial mutualism.
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DOI：
10.1371/journal.pone.0017105
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发表时间：
2011-02-16
期刊：
影响因子：
3.7
通讯作者：
Yomo T
中科院分区：
文献类型：
--
作者：
Hosoda K;Suzuki S;Yamauchi Y;Shiroguchi Y;Kashiwagi A;Ono N;Mori K;Yomo T
为了了解两个以前没有接触过的生物如何建立互利共生，首先有必要检查它们在互利共生建立过程中表型的时间变化。我们没有追溯已知的、成熟的、自然的互利共生的历史，而是用两种基因工程的营养缺陷型大肠杆菌菌株模拟了互利共生的发展，这两种菌株模仿了两种以前从未见过的生物，但后来建立了互利共生。在这种合成互利共生的发展过程中，一种菌株在与伴侣菌株相遇约10小时后，开始过度供应伴侣生长所必需的代谢物，最终导致两种菌株的连续生长。这种合作表型适应性地出现后，遇到的合作伙伴菌株，但之前的菌株本身的增长。通过转录组分析，我们发现，该菌株的合作表型并不伴随着局部激活的生物合成和运输的过度供应的代谢产物，而是由全球激活的合成代谢。这项研究表明，一个生物体有潜力适应其表型后，第一次遇到另一个生物体建立互利共生之前，它的灭绝。由于不同的生物在自然界中不可避免地会遇到彼此，这种潜力将在自然界中建立新生的互利共生关系方面发挥重要作用。
To understand how two organisms that have not previously been in contact can establish mutualism, it is first necessary to examine temporal changes in their phenotypes during the establishment of mutualism. Instead of tracing back the history of known, well-established, natural mutualisms, we experimentally simulated the development of mutualism using two genetically-engineered auxotrophic strains of Escherichia coli, which mimic two organisms that have never met before but later establish mutualism. In the development of this synthetic mutualism, one strain, approximately 10 hours after meeting the partner strain, started oversupplying a metabolite essential for the partner's growth, eventually leading to the successive growth of both strains. This cooperative phenotype adaptively appeared only after encountering the partner strain but before the growth of the strain itself. By transcriptome analysis, we found that the cooperative phenotype of the strain was not accompanied by the local activation of the biosynthesis and transport of the oversupplied metabolite but rather by the global activation of anabolic metabolism. This study demonstrates that an organism has the potential to adapt its phenotype after the first encounter with another organism to establish mutualism before its extinction. As diverse organisms inevitably encounter each other in nature, this potential would play an important role in the establishment of a nascent mutualism in nature.
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影响因子：
2
作者：
HAMILTON, WD
通讯作者：
HAMILTON, WD
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
56.9
作者：
Chuang, John S.;Rivoire, Olivier;Leibler, Stanislas
通讯作者：
Leibler, Stanislas
影响因子：
56.9
作者：
BUCHSBAUM, RALPH;BUCHSBAUM, MILDRED
通讯作者：
BUCHSBAUM, MILDRED
DOI：
10.1073/pnas.222313099
发表时间：
2002-11-12
影响因子：
11.1
作者：
Aanen, DK;Eggleton, P;Boomsma, JJ
通讯作者：
Boomsma, JJ