In vivo STED microscopy visualizes PSD95 sub-structures and morphological changes over several hours in the mouse visual cortex.
In vivo STED microscopy visualizes PSD95 sub-structures and morphological changes over several hours in the mouse visual cortex.
复制标题
DOI：
10.1038/s41598-017-18640-z
复制
发表时间：
2018-01-09
影响因子：
4.6
通讯作者：
Willig KI
中科院分区：
文献类型：
--
作者：
Wegner W;Mott AC;Grant SGN;Steffens H;Willig KI
突触后密度（PSD）是一个电子致密区，由约1000种蛋白质组成，位于兴奋性突触的突触后膜上，其大小取决于突触强度。PSD 95是PSD中丰富的支架蛋白，并组装由神经递质受体、离子通道以及信号和结构蛋白组成的超复合物家族。我们使用超分辨率STED（STimulated Emission Depletion）纳米显微镜来确定麻醉小鼠视觉皮层中PSD 95的大小和形状。表达与内源性PSD 95蛋白融合的eGFP的成年敲入小鼠在1分钟至6小时的时间点成像。PSD 95的超分辨大组件显示出不同的子结构;大多数大组件是环状的，一些是马蹄形或8字形，形状是连续的或由纳米团簇组成。子结构在较短（分钟）时间点内显得稳定，但1小时后，超过50%的大组装体显示出子结构的变化。总之，这些数据显示了在麻醉小鼠中观察6小时内发生变化的大PSD 95组装体的亚形态。
The post-synaptic density (PSD) is an electron dense region consisting of ~1000 proteins, found at the postsynaptic membrane of excitatory synapses, which varies in size depending upon synaptic strength. PSD95 is an abundant scaffolding protein in the PSD and assembles a family of supercomplexes comprised of neurotransmitter receptors, ion channels, as well as signalling and structural proteins. We use superresolution STED (STimulated Emission Depletion) nanoscopy to determine the size and shape of PSD95 in the anaesthetised mouse visual cortex. Adult knock-in mice expressing eGFP fused to the endogenous PSD95 protein were imaged at time points from 1 min to 6 h. Superresolved large assemblies of PSD95 show different sub-structures; most large assemblies were ring-like, some horse-shoe or figure-8 shaped, and shapes were continuous or made up of nanoclusters. The sub-structure appeared stable during the shorter (minute) time points, but after 1 h, more than 50% of the large assemblies showed a change in sub-structure. Overall, these data showed a sub-morphology of large PSD95 assemblies which undergo changes within the 6 hours of observation in the anaesthetised mouse.
登录
查看更多内容
影响因子：
4.6
作者：
Broadhead MJ;Horrocks MH;Zhu F;Muresan L;Benavides-Piccione R;DeFelipe J;Fricker D;Kopanitsa MV;Duncan RR;Klenerman D;Komiyama NH;Lee SF;Grant SG
通讯作者：
Grant SG
影响因子：
4.8
作者：
Keith, Dove;El-Husseini, Alaa
通讯作者：
El-Husseini, Alaa
影响因子：
16.2
作者：
Opazo, Patricio;Labrecque, Simon;Choquet, Daniel
通讯作者：
Choquet, Daniel
影响因子：
2.9
作者：
Herculano-Houzel S;Watson C;Paxinos G
通讯作者：
Paxinos G
树突状棘的超微结构：突触和脊柱形态之间的相关性。
影响因子：
4.3
作者：
Arellano JI;Benavides-Piccione R;Defelipe J;Yuste R
通讯作者：
Yuste R